摘要
从山东省棉田自然死亡的棉铃虫上分离到常见致病菌至少有8种,经鉴定为:苏云金杆菌(Bacillusthuringiensis)、蜡状芽孢杆菌(Bacillus cereus)、铜绿假单胞菌(Pseudomonas aeruginosa)、球孢白僵菌(Beauveriabassiana)、卵孢白僵菌(B.tenella)、青霉菌属真菌(Penicilliumspp.)、拟青霉属真菌(Paecilimycesspp.)和蛾霉属真菌(Nomuraespp.);得到一株对棉铃虫高毒的苏云金杆菌菌株Hu,其毒力比普遍使用的Bt菌系7216高,二者的LC50分别为15.1μg/mL和26.28μg/mL。蛾霉属真菌对棉铃虫有明显的拒食作用和杀伤作用,但人工培养时生长十分缓慢。
Eight species of pathogenic microbes isolated from larvacs of Helicoverpa armigera naturally diseased and collected from cotton fields in Shandong Province were identified. They were Bacillus thuringiensis, Bacillus cereus, Pseudomonas aeruginosa, Beauveria bassiana, Beauveria tenella, Penicillium spp., Paecilimyces spp. and Nornurae spp. Among them, a Bacillus thuringiensis isolate numbered Hu was the most virulent to Helicoverpa armigera based on bioassay. Toxicity of Hu to H. armigera lavae was higher than that of Bt7216 which was commonly used in biopesticide plants, with LC50 of Hu and Bt7216 were 15.1 μg/mL and 26.28μg/mL respectively. Nomurac spp. had an antifeedant effect and was pathogenic to H. armigera, but was slow in growth on PDA.
出处
《山东科学》
CAS
2006年第6期1-5,共5页
Shandong Science
关键词
致病菌
棉铃虫
死亡率
分离
鉴定
筛选
insect pathgen
Helicoverpa armigera
morality
isolation
identification
screening
