摘要
目的从胃癌发生发展的角度探讨胃蛋白酶原C（PGC）基因插入-缺失多态与胃癌及其癌前疾病易感性的关系，并初步探讨PGC基因多态与H.pylori感染的交互作用对胃癌发生发展的影响。方法利用聚合酶链反应（PCR）及琼脂糖电泳分析方法，检测141例胃癌患者和564例非胃癌者PGC基因的基因型；比较不同基因型与胃癌和胃癌前疾病的关系。利用酶联免疫吸附法（ELISA）检测血清H.pylori-IgG滴度，并以效应修饰模型分析PGC—H.pylori感染之间的交互作用。结果PGC等位基因1纯合型与非纯合型携带者比较，纯合型携带者罹患萎缩性胃炎的风险增加到3．103倍（95％CI，1．440～6．686），罹患胃癌的风险增加到2．962倍（95％CI，1．370～6．404），发生肠上皮化生的风险增加到1.659倍（95％CI，0.998～2．757）。携带等位基因1纯合型并且pylori-IgG阳性的个体，罹患萎缩性胃炎和胃癌的风险显著增高（似然比检验，P=0.023和P=0.005）。结论PGC基因多态与萎缩性胃炎、胃癌的遗传易感性有关。PGC基因多态与H.pylori感染在胃癌及其癌前疾病的发病过程中存在交互作用。
Objective To assess the relationship between the insert-deletion polymorphism in the Human pepsinogenc （PGC） gene and susceptibility to gastric cancer, together with its precursors, and to investigate the interaction between PGC polymorphism and H. pylori infection in the development of gastric cancer. Methods PGC gene polymorphism in 141 patients with gastric cancer and 564 matched non-cancer controls were detected by polymerase chain reaction （PCR）, and the relation between the PGC polymorphism and gastric cancer was examined. Serum H. pylori-IgG was determined with ELISA method. The odds ratios and 95% confidence intervals （95% CI） were calculated using unconditional logictic regression model. The effect-modified model was used to evaluate the PGC-H. pylori interaction. Results No significant association between the frequency of homogenous allele 1 of PGC gene and the sex and age of the subjects was observed. The subjects with the homogenous allele 1 had an increased risk of developing atrophic gastritis （OR： 3. 103, 95%CI： 1. 440-6. 686）, and gastric cancer（OR： 2. 962,95% CI： 1. 370 6. 404）, and Intestinal metaplasia （ OR： 1. 659,95% CI： 0. 998-2. 757） comparing with those with the non- homogenous allele 1. For subjects carrying both homogenous allele 1 and H. pylori-IgG positive, there was an significant increased risk of developing atrophic gastritis and gastric cancer （ likelihood test ratio test ： P = 0. 023, P = 0. 005 ）. Conclusion PGC gene polymorphism may be associated with susceptibility to gastric cancer and atrophic gastritis. The PGC gene polymorphism and H. pylori infection seem to display an interaction in the development of gastric cancer.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2006年第43期3059-3063,共5页
National Medical Journal of China
基金
国家自然科学基金资助项目(30572131)
关键词
胃蛋白酶原C
胃肿瘤
基因
螺杆菌
幽门
Pepsinogen C
Stomach neoplasms
Gene
Helicobacter pylori
