摘要
目的构建缺血性脑损伤大鼠脑组织芯片,为缺血性脑损伤研究提供实验工具。方法将50只雄性SD大鼠随机分为脑缺血20 m in后再灌注1、4、7、14、28 d五组,每组8只,每个时间点设立假手术组2只。采用四血管闭塞全脑缺血模型。选择大脑颞叶皮质、海马、胼胝体为观察位点,依次经过微阵列设计、预制受体蜡块、供体蜡块定位、穿芯取样、连续切片及烤片等步骤,构建缺血性脑损伤大鼠脑组织芯片。结果在36 mm×26 mm×15 mm受体蜡块上,构建15×10共150点阵芯片,分布150个组织标本,每点直径1.0 mm,并连续4μm厚微阵列切片。肉眼所见点阵分布均匀,蜡块无裂纹,载玻片上点阵无脱落,有6点发生移位,移位率为4%。HE染色显示取样组织边缘清晰,无明显组织结构变形。结论成功构建大鼠缺血脑组织芯片,为缺血性脑损伤研究提供便捷的实验工具。
Objective To construct tissue microarrys in rats with ischemic brain injury and to provide an experimental means for the study of isehemie brain injury. Methods Fifty male Sprague-Dawley rats were randomly assigned to cerebral isehemia 1-, 4-, 7-, 14- and 28-day groups, and each group had 8 rats. Sham-operation group （2 rats） was established at each time points. The model of global four-vessel occlusion was used. Cortex of temporal lobe, hippoeampus and corpus callosum were selected as observing points. The tissue microarrys in ischemic brain injury rats were constructed in the order of designing microarrys, prefabricating recipient paraffin blocks, localizing donator paraffin blocks, sampling, slicing continuously and baking slice, etc. Results One hundred and fifty lattice arrays with 15 ×10 were constructed on 36 mm × 26 mm × 15 mm recipient paraffin blocks, and 150 tissue specimens were distributed, each lattice was 1.0 mm in diameter, and the microarrys were cut into 4 μm per layer. The lattice lattices distributed evenly under macroscopic observation, there were no fissuarings on paraffin blocks, and the point lattices did not fall off on microscope slide. Six point lattices displaced, and the displacement rate was 4%. HE stain showed sharp margin surrounding sampling tissues without obvious tissue crushing and deformation. Conclusion The successful construction of tissue microarry may provide a convenient and efficient experimental means for the study of ischemic brain injury..
出处
《中国脑血管病杂志》
CAS
2006年第11期504-507,共4页
Chinese Journal of Cerebrovascular Diseases
关键词
组织芯片
脑缺血
大鼠
Tissue microarray
Cerebral ischemia
Rats
