摘要
目的探讨自噬基因Beclin 1在宫颈鳞癌中的蛋白表达以及与肿瘤发生发展的关系。方法采用免疫组化SP法检测81例宫颈鳞癌、20例宫颈上皮内瘤样病变（CIN，Ⅱ～Ⅲ级）和20例正常宫颈组织中自噬基因Beclin 1的蛋白表达。结果宫颈鳞癌组织中Beclin 1阴性、弱阳性、强阳性表达率依次是43．2％（35／81）、34．6％（28／81）、22．2％（18／81），与正常宫颈和宫颈上皮内瘤样病变组相比，Beclin 1在宫颈鳞癌中的表达显著下调（P=0．011）。宫颈鳞癌中Beclin 1的表达在不同FIGO分期、年龄、宫颈肌层浸润深度、宫颈肿瘤大小、大体类型间的差异无统计学意义。但在有无盆腔淋巴结转移、不同肿瘤分化程度间差异有统计学意义（P〈0．05）。结论自噬基因Beckn 1在宫颈鳞癌中蛋白表达下调，并与宫颈鳞癌盆腔淋巴结转移和肿瘤分化程度有关。
Objective Autophagy gene Beclin 1 plays an important role in several types of human cancers. So we, in this study, employed the immunohistochemical manner to detect the Beclin 1 gene expression and explore its clinical significance in cervical aquamous cell carcinoma. Metheds SP immunohistochemistry technique was used to detect the expression of Beclin 1 gene in specimens of 81 cervical squamous cell carcinoma, 20 cervical intraepithelial neoplasm （CIN, Ⅱ -Ⅲ）, and 20 normal cervix. Correlations between the expressions of Beclin 1 gene and the clinicopathologic factors of cervical squamous cell carcinoma were statistically analyzed. Results The rates of negative, weak, strong expression of Beclin 1 in cervical squamous cell carcinoma were 43.2% （35/81）, 34. 6% （28/81） or 22.2% （18/81） respectively, and the significantly lower than those in normal cervix and CIN Ⅱ - Ⅲ （P= 0. 011）. The Beclin 1 expressions did not associate with FIGO stage, age, depth of cervical infiltration, tumor size, and gross type of cervical lesion （P〈0. 05）, but were related to pelvic lymph node metastases and histological tumor grade （P〈0.05）. Conclusions Expression of autophagy gene Beclin 1 decreases in cervical aquamous cell carcinoma, and is closely related to pelvic lymph node metastases and histological tumor grade.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2006年第6期860-863,共4页
Journal of Sichuan University(Medical Sciences)
关键词
宫颈鳞癌
自噬
BECLIN
1免疫组化
Cervical squamous neoplasm Autophagy Beclin 1 Immunohistochemistry
