摘要
目的:建立石墨炉原子吸收分光光度法测定人尿中砷含量。方法:使用镀层石墨管、塞曼背景校正,用氯化钯、高锰酸钾和 Triton X-100处理样品,在193.7nm 波长处测定。结果:测定尿砷浓度的线性范围10～100ng·mL^(-1)(r=0.9993),定量下限10ng·mL^(-1);石墨管管内 RSD 为6.23%～4.74%,管间 RSD 为6.30%～4.26%;方法回收率99.60%～102.9%;20,40,100ng·mL^(-1)质控 RSD 为10.63%,7.88%,5.57%;测定了158名住院病人尿砷含量,75.95%的尿样砷含量低于定量下限,砷含量最高的样品浓度为268.4ng·mL^(-1)。结论:本方法简便、可靠,适用于临床尿砷含量测定和筛查。
Objective：To establish an atomic absorption spectroscopy method to determinate concentration of arsenic in human urine. Method：The analysis was carried on plating graphite tube,with zeeman background correction. We used PdCl2, KMnO4 and Triton X - 100 to deal with sample, and determined under 193.7 nm. Results：The linear range was 10 - 100 ng · mL^-1 （ r = 0. 9993 ） , the limit of quantitation was 10 ng · mL ^- 1, intra - tube RSD was 6. 23% -4.74% ,inter - tube RSD was 6. 30% -4.26%, recovery rate was 99.60% - 102.9%, RSD of quality control by 20,40,100 ng · mL^-1 was 10.63% ,7.88% ,5.57% ;we used this method on 158 patients＇ urine samples, the arsenic concentration in 75.95% samples was below limit of quantitation （10 ng · mL^-1 ）, the highest arsenic concentration was 268.4 ng · mL^-1. Conclusion：This method is rapid, reliable and suitable for clinical samples screening.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第10期1441-1444,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
石墨炉原子吸收分光光度法
尿砷含量测定
graphite furnace atomic absorption spectrometer
determination of arsenic in human urine
