摘要
采用地高辛标记生长抑素反意ＲＮＡ探针经原位杂交和显色后，光学显微镜下观察生长抑素ｍＲＮＡ在大鼠脊髓内的定位。结果显示：脊髓内含有大量呈紫蓝色的生长抑素ｍＲＮＡ阳性神经细胞，碱性磷酸酶反应产物定位于胞浆内，胞核不着色，生长抑素ｍＲＮＡ阳性胞体主要分布于脊髓背角Ⅱ层，Ⅲ Ⅳ、Ｖ和Ｘ层内有少量生长抑素ｍＲＮＡ阳性胞体，Ⅶ和Ⅷ层内仅偶见生长抑素ｍＲＮＡ阳性胞体。
In situ hybridization of somatostatin mRNA in the rat spinal cord was observed at light microscopic level with digoxengenin-labelled somatostatin antisense RNA as the probe. We visualized numerous purple-blue somatostatin mRNA-positive neurons in the spinal cord. Alkaline phosphatase reaction products were concentrated in the cell cytoplasm. The nucleus was unlabelled. A lot of somatostatin mRNA-positive neurons were found in layer Ⅱ and a few in layers Ⅲ, Ⅳ, V, X and occasional in layers Ⅶ,Ⅷ.
出处
《动物学报》
SCIE
CAS
CSCD
1996年第3期244-246,共3页
ACTA ZOOLOGICA SINICA
关键词
大鼠
脊髓
生长抑素
MRNA
原位杂交
Rat, Spinal cord, Somatostatin mRNA, In situ hybridization.
