摘要
采用典型产氢途径的丁酸梭菌和阴沟肠杆菌作为对比菌株,铁元素作为限制性因子,研究Ethanoligenens sp.B49的产氢代谢过程对铁的依赖性以及产氢机制.铁限制性培养结果表明,与完全培养条件相比,在铁浓度限制在2mg.L-1条件下B49仍保持很好的生长状态和产氢性能,其产氢不受铁元素不足的影响,即其产氢行为不具有铁依赖性;这一性质与梭菌型产氢途径的丁酸梭菌表现出相似的规律,完全区别于阴沟肠杆菌铁依赖型的产氢机制.初步判断高效产氢菌B49的产氢代谢途径类似于梭菌型产氢途径.
The hydrogen-producing pathway of Ethanoligenens sp, B49 was investigated, As a metabolic limiting factor, the influence of iron on the hydrogen production in the batch culture fermentation process was evaluated. Besides, Clostridium butyricum and Enterobacter cloaca which have typesof typical hydrogen-producing pathways were adopted for comparison with strain B49. The results showed that the activity and hydrogen-producing capacity of strain 1349 varied little under the conditions of adding and without adding iron, which implied that hydrogen-producing behavior of Ethanoligenens sp. B49 did not depend on iron. This characteristic was similar to that of Clostridium butyricum but quite different from Enterobacter cloaca with Coli-type hydrogenproducing pathway. Therefore, the hydrogen production by Ethanoligenens sp, B49 was probably by Clostridial type pathway.
出处
《环境科学学报》
CAS
CSCD
北大核心
2006年第10期1643-1650,共8页
Acta Scientiae Circumstantiae
基金
国家自然科学基金(No.30470054)~~
关键词
产氢细菌
新种
铁依赖性
代谢途径
发酵
hydrogen-producing bacteria
novel strain
iron
metabolic pathway
fermentation
