摘要
盐渍胁迫往往抑制植物种子萌发与幼苗生长。如何促进盐胁迫下种子萌发是盐渍土地区作物栽培的重要技术环节。以西瓜为材料,研究了不同浓度NaCl处理对种子萌发的影响,发现在125mmol/LNaCl处理下添加15～30mg/L外源5-氨基乙酰丙酸(ALA)可以明显缓解盐胁迫对种子萌发和幼苗生长的抑制效应,并提高幼苗超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)以及过氧化物酶(POD)活性,降低丙二醛(MDA)含量和脂氧合酶(LOX)活性。此外,利用POD活性抑制抗坏血酸(AsA)处理,则可完全抑制ALA对种子发芽的促进效应。以上结果表明,外源ALA能够促进盐胁迫下西瓜种子萌发,并且可能与ALA诱导抗氧化酶,特别是POD活性的提高有关。
Salinity usually prohibits seed germination and seedling growth. How to promote seed germination and seedling growth has been an important agricultural technique in saline regions. In the work, it was found that 75 to 125mmol/L NaCl treatment significantly inhibited seed germination and seedling growth of watermelon. Treatment with 15 to 30 mg/L 5- aminolevulinic acid （ALA） could promote seed germination and seedling growth under 125 mmol/L NaCl stress, companied with the increased activities of superoxidate dismutase （SOD）, ascorbate peroxidase （APX） and peroxidase （POD）, but the decreased activity of lipoxygenaze （LOX） and a lower level of MDA in hypocotyls and radicles. Addition with ascorbic acid （AsA）, an inhibitor of POD activity, in a concentration of 1 g/L might eliminate the promotion of ALA on seed germination, which suggested that the promotion of exogenous ALA treatment on germination under salt stress might be associated with the increased activities of the antioxidant enzymes, especially POD.
出处
《果树学报》
CAS
CSCD
北大核心
2006年第6期854-859,共6页
Journal of Fruit Science
基金
国家自然科学基金项目(30471181)。
关键词
西瓜
5-氨基乙酰丙酸
盐胁迫
种子萌发
抗氧化酶
Watermelon
5-Aminolevulinic acid （ALA）
Salt stress
Seed germination
Antioxidant enzymes
