摘要
目的建立比溴化乙锭(EB)更为安全的核酸染色方法。方法对SYBR Gold,SYBR GreenⅠ,Gold-View和EB等4种核酸荧光染料在琼脂糖凝胶中的染色特性进行比较分析。结果SYBR Gold和SYBR GreenⅠ可改变核酸的电泳迁移率及相应的DNA大小判断,但二者是比EB更为完全和经济的核酸荧光染料;对于小片段DNA,SYBR Gold的灵敏度高于SYBR GreenⅠ。结论SYBR Gold和SYBR GreenⅠ是比EB更为完全和经济的核酸荧光染料,适合于分子生物学实验室的常规应用。
OBJECTIVE To set-up safe alternatives to ethidium bromide （EB）. METHODS Comparative analysis was performed on the DNA staining efficiencies of 4 fluorescent dyes including SYBR Gold, SYBR Green I , GoldView and EB in preparative agarose gel electrophoresis. RESULTS Although both SYBR Gold and SYBR Green I ahered electrophoretic mobility and thus DNA size estimates, they were cost-effective ahernatives to EB. SYBR Gold was more sensitive than SYBR Green I at detecting short fragments, but 50 bp bands were clearly visible using either dye when visualized with a long integration time. CONCLUSIONS SYBR Gold or SYBR Green I are sensitive and relatively safe alternatives to EB.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2006年第11期1316-1318,共3页
Chinese Journal of Nosocomiology
基金
国家自然科学基金资助(30370398)
重庆市自然科学基金资助(2004-27-22CSTC-2005BB5297)
关键词
核酸染色
溴化乙锭
荧光染料
Nucleic acid staining
Ethidium bromide
Fluorescent dyes
