摘要
采用瑞士CAMAG Scanner 3薄层扫描仪对黄姜中的薯蓣皂苷元进行测定,以V（石油醚）：V（氯仿）：V（甲醇）＝10：10：0.5为展开剂,用20 g/L磷钼酸无水乙醇溶液喷雾显色,140 ℃烘烤约5 min,检测波长650 nm,狭缝尺寸为6.00 mm×0.30 mm.结果表明：薯蓣皂苷元在0.3～1.8 μg的范围内与峰面积呈良好线性关系,相关系数r=0.9983,回收率为96.94% （n=5）.
A method was developed to determine the content of diosgenin in Disocoreo zingiberensis by CAMAG TLC SCANNER 3 thin layer chromatography scanning. The developing solvent is composed of petroleum ether-chloroformMethanol （10： 10：0.5）. Layers were baked for 5 rain at 140 ℃using phospho-molybdic-dehydrated alcohol solution （2%） as chromogenic reagent. The detection wavelength is 650 nm and the slit size 6.00 mm× 0.30 mm. The results showed that the linearity of the calibration curve was good in the range of 0.3 - 1.8 μg （r = 0. 9983）, and the average recovery （ n = 5） was 96.94 % for diosgenin.
出处
《分析试验室》
CAS
CSCD
北大核心
2006年第11期67-69,共3页
Chinese Journal of Analysis Laboratory
基金
河南省自然科学基金(0511020200)项目资助
