摘要
目的为了探讨细胞因子在哮喘发病中的作用，寻找新的治疗途径。方法采用重组白细胞介素－４（ｒＩＬ－４）和γ－干扰素（ＩＦＮ－γ）对３０例哮喘患者及２５名正常人作对照，进行外周血单个核细胞（ＰＢＭＣ）体外ＩｇＥ合成调控的研究。结果哮喘组ＰＢＭＣ体外自发合成ＩｇＥ明显高于对照组（ｔ＝４．４７１２，Ｐ＜０．００１）。经ｒＩＬ－４刺激后ＰＢＭＣ合成ＩｇＥ显著升高。ｒＩＬ－４诱导ＩｇＥ合成的程度哮喘组明显低于对照组（ｔ＝４．６７１９，Ｐ＜０．００５）。ＩＦＮ－γ不仅抑制ＰＢＭＣＩｇＥ的自发合成，而且抑制ｒＩＬ－４的诱导合成。ＩＦＮ－γ抑制ＰＢＭＣＩｇＥ合成的程度哮喘组大于对照组（ｔ＝７．１８３３，Ｐ＜０．００１）。
ObjectiveInordertoelucidatethecytokinesresponsibleforalteredIgEproducinginpatientswithasthmaMethodsTheinvitroeffectsofrecombinantinterleukin-4(rIL-4)andinterfer-on-γ(IFN-γ)onsynthesisofIgEbyperipheralbloodmononuclearcel(PBMC)werestudiedResultsSpon-taneousIgEsynthesisbyPBMCwassignificantlyincreasedinpatientswithasthmacomparedwiththecontrols(t=4.4712,P<0.001)AlthoughthemaximumamountsofIgEsynthesisusingPBMCafterstimulationwithrIL-4werealmostthesamebothinpatientswithasthmaandincontrols(t=0.0620,P>0.05),theenhancementrateofrIL-4-inducedIgEsynthesiswaslowerinasthmaticpatientsthaninthecontrols(t=4.6719,P<0.05)IFN-γsuppressednotonlyspontaneousbutalsorIL-4-inducedIgEsynthesisbyPBMCinasthmaticpatients.ThesuppressiveeffectonIgEsynthesiswasmoreprominentinpatientswithasthmathaninthecontrols(t=7.1833,P>0.001)ConclusionsTheseresultssuggestthatasthmaatacksarerelatedtoIgE-dependenttriggermechanism;theim-balanceofIL-4andIFN-γinvivoisinvolvedinthedevelopmentofasthma.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
1996年第5期286-289,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
