摘要
利用毛细管电泳（ｃａｐｉｌｌａｒｙｅｌｅｃｔｒｏｐｈｏｒｅｓｉｓ，ＣＥ）分离猪脑、鼠脑组织中主要几种神经节苷脂ＧＭ_１、ＧＤ_（１ａ）、ＧＤ_（１ｂ）、ＧＴ_（１ｂ），在缓冲液中加入α－环状糊精（α－ｃｙｃｌｏｄｅｘｔｒｉｎ，α－ＣＤ）来提高分离的选择性和效率，并对缓冲液的ｐＨ值、α－ＣＤ浓度和电压等变化因素进行研究。结果表明在含１５．０ｍｍｏｌ／Ｌα－环状糊精的２．５ｍｍｏｌ／Ｌ磷酸级冲液（ｐＨ８．６）中，电压为２５ｋＶ时能很好的分离生物样品。从猪脑提取物中分离出ＧＭ_３、ＧＭ_１、ＧＤ_（１ａ）、ＧＤ_（１ｂ）、ＧＴ_（１ｂ）和含唾液酸的其他糖脂类物质，鼠脑中分离出ＧＭ_１、ＧＤ_（１ａ）和ＧＴ_（１ｂ），说明毛细管电泳可用于分离神经节苷脂，具有快速、简便、节约等特点。
Gangliosides are glycosphingolipds containing sialic acid. They have been demonstrated to play important roles in biological functions,such as cell growth,differentiation,malignant transformation, membrane-mediated transfer of information. We have separated several major gangliosides from pig brain and rat brain by capillary electrophoresis (CE). Enhancement of selectivity and efficiency of separation were obtained by using 2. 5 mmol/L phosphate buffer containing 15 mmol/L a-cyclodextrin. We also studied the factors which effect on the separation of the samples. The results indicate that the methods of using capillary electrophoresis can successfully separate gangliosides GM1,GM3,GD1a,GD1b, GT1b from pig brain and GM1,GD1a,GT1b, from rat brain.
出处
《首都医科大学学报》
CAS
1996年第3期183-188,共6页
Journal of Capital Medical University
关键词
脑
神经节苷脂
毛细管电泳
哺乳动物
pig brain
rat brain
gangliosides
capillary electrophoresis
