摘要
目的为制备FVB转基因小鼠及保种繁殖提供最佳超排周龄及胚胎冷冻方法。方法对4—6周、6．8周、8．10周FVB小鼠进行超数排卵效果的比较及采用体外培养受精卵至2-cell法和输卵管吹卵法两种不同方法收集2-cell受精卵进行胚胎冷冻。结果4．6周雌鼠经超排后回收的受精卵（24．6个）明显高于6—8周龄、8—10周龄的FVB雌鼠（14．5个、16．2个），差异有显著性；两种方法回收的2-cell受精卵经冷冻保存后，Ⅱ组受精卵解冻后2-cell形态正常的胚数（106个）高于I组解冻后2-cell形态正常的胚数（90个），但两者无显著性差异。结论本研究将为制备FVB转基因小鼠及转基因动物的种系保存和繁殖提供了基础。
Objective To provide the best time of superovulation and the best method of embryo freezing for making FVB transgenic mice and breeding. Methods Using the means of culturing amphicytula in vitro to 2-cell and tubal insuffiations to collect the 2-cell amphicytulas, then did embryo freezing, and compared the effect of superovulation of FVB mouse which live for 4 - 6weeks, 6 -8weeks, 8 - 10 weeks. Result The amphicytulas of the female mouse which lived for 4 - 6 weeks were retrieved after superovulation, the quantity （24.6）of them was much more than those from FVB female mouse which lived for 6 - 8 weeks and 8 - 10 weeks（ 14.5,16.2）, there was significant difference between the female mouse which lived for 4- 6weeks and 6 - 8weeks, 8 - 10 weeks After cryopreservation of the 2-cell amphicytulas retrieved by the 2 methods, the quantity of 2-cell amphicytulas with normal morphous from the group Ⅱ （106）was more than that from group Ⅰ （90）after defrosting, but the difference was not significant. Conclusion The research provided the foundation for making transgenic mice of FVB and breeding them.
出处
《中国比较医学杂志》
CAS
2006年第11期652-654,共3页
Chinese Journal of Comparative Medicine
基金
辽宁省科技计划项目(2004408001)
关键词
小鼠
超排卵
胚胎冷冻
Mice
Superovulation
Embryonic freezing
