摘要
将制备的L-半胱氨卟啉铜(Ⅱ)(CuL)配合物自组装在Au电极表面,获得电化学苯酚传感器(CuL/AuCME)。在pH7.0的磷酸盐缓冲液中于-0.4～0.5V(vs.SCE)电位范围内有一对氧化还原峰,其峰电位分别为氧化峰电位Epa=90mV,还原峰电位Epc=-60mV。实验表明该电极上,苯酚可被CuL催化氧化,通过产物在电位0.1V下的电化学响应对苯酚进行测定,测定过程不需要再向体系中添加媒介体。该电极对苯酚表现出快速的响应(响应时间<10s)。传感器对苯酚的测定具有较宽的线性范围(5×10-7～2.5×10-4mol/l),检出限为2.0×10-7mol/l。测定了电极稳定性,将该电极用于地表水中苯酚含量检测,并与标准4-氨基安替比林分光光度法作了对照,结果满意。
A novel amperometric phenol sensor was constructed through the covalent binding of L-cysteamine tailed porhyrin Cu（Ⅱ） （CuL） on a gold electrode by using the self-assembly technique. The electrode shows good electrochemical activity with one pair of redox peaks （Epa=90mV, Epc=-60V ） in pH7.0 PBS between -0.4- 0.SV（vs.SCE）. The experiment shows that phenol could be oxidized by immobilized CuL to form a detectable product which was determined at 100 mV without any mediator. The phenol sensor exhibited a fast response （less than 10 seconds）. The linear range for phenol determination was from 5.0×10^-7 to 2.5×10^-4 mol/l with a detection limit of 2.0×10^-7 mol/l. The stability of the biosensor was also evaluated and shows fairly stable. It＇s applied for the determination of phenol in real natural waters and compared with the routine 4-aminopyrine spectro-photometry. The results are satisfactory.
出处
《传感器世界》
2006年第10期11-15,共5页
Sensor World
基金
国家自然科学基金(No:60508012)
浙江省科技攻关项目(No:2006C31040)资助项目。
