摘要
以银杏无菌苗带芽的茎段、幼叶,成熟胚的子叶、根段,雌、雄株嫩茎、雄花粉等为外植体,研究了它们在不同培养上的器官分化情况。结果表明,无菌幼苗茎段可以诱导出双芽体,雌、雄株的当年生嫩茎可以诱导出双芽体或3芽体,不定芽的发生为直接再生;子叶在MS+NAA1.0mg/L+KT2.0mg/L培养基上诱导出2个不定芽,小苗可长至2.0cm。另外有些子叶在切口基部产生白不定根。30d叶龄的无菌幼苗叶片在MS+NAA1.0mg/L+BA1.0mg/L或MS+NAA1.0mg/L+KT0.5mg/L上,有直接发生不定根现象,最高生根率达40%;雄花粉也有不定根的发生。
An experiment was conducted to study the organ differentiation of Ginkgo biloba on different media using stem sections with bud and young leaves of aseptic seedlings, cotyledon and roots of mature embryos, annual twigs of female and male plants, and male pollens as explants. Result showed that the young stems from the aseptic seedlings induced 2 adventitious buds, while those from mature plants developed 2 or 3 adventitious buds, and the adventitious buds were directly regenerated. 2 adventitious buds were observed on cotyledons cultured on MS ＋ NAA1.0 mg/L ＋ KT2.0 mg/L, and the plantlet could grow as high as 2 cm. Differentiated adventitious roots were induced from the 30-day-old leaves of aseptic seedlings on MS ＋ NAA1.0 mg/L ＋ BA1.0 mg/L and MS ＋ NAA1.0 mg/L ＋ KT0.5, and the highest rooting rate reached 40 percent. Adventitious roots could also develop on young leaves and immature male pollens.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2006年第6期4-6,12,共4页
Journal of Northeast Forestry University
基金
高等学校博士学科点专项科研基金项目(20050298008)。
关键词
银杏
外植体
器官分化
Ginkgo biloba
Explants
Organ differentiation
