摘要
本文用反相高效液相色谱(HPLC)法测定头孢氨苄的尿药浓度。尿样只需经微孔滤膜过滤即可进样,采用μ-Bondapack C-18反相色谱柱,流动相为甲醇-水(含醋酸-醋酸铵缓冲对,pH=3.91),紫外254nm处检测,保留时间9min45s。口服头孢氨苄7.5h后肾功正常者排出93.7％,肾功不全者只排出54.8％。肾功不全者用消除速度常数调整剂量与用肌酐清除率调整,结果相近。
The assay of cephalexin involves micro-filtration of urine specimens followed by HPLC on a μ-Bondapack C-18 reverse phase column utilizing a mobile phase of MeOH-H2O containing HAc-NH4Ac (pH = 3.91 ) .Its quantitation by UV response at 254 nm covered a wide range of concentration down to lμg/ml and no metabolite peaks were detected.The retention time was 9 min 45 sec under our condition. 7.5 h after oral administration, the excretion of cephalexin is 93.7% in no~ rmal subjects while about 54.8% in patients with impaired renal function.Oral dose of the patients adjusted by Ke is similar to that by Ccr.
出处
《第一军医大学学报》
CSCD
1990年第2期176-179,共4页
Journal of First Military Medical University
关键词
头孢氨苄
HPLC
肾功能不完
HPLC
cephalexin
dosage adjustment
renal function
