摘要
作者用碘^(125)标记由顺-乌头酸酐法制备的胃癌单抗(MGB_2)-柔红霉素(DM)结合物,观察其在荷人胃癌SGC-7901裸鼠体内分布状态.在MGB_2—DM中,每个抗体分子引入4～5个分子DM,抗体活性保存良好.结果表明,^(125)Ⅰ—MGB_2—DM能较满意地定位于肿瘤组织,腹腔给予^(125)Ⅰ-MGB_2 DM 96h,瘤/血比值达203,其结果与未修饰MGB_2相近.
In the present study, an and gastric cancer monoclonal antibody MGb2 daunomycin (DM) conjugate was prepared with cis - acomtic anhydride method The distribution of the conjugate in nude mice bearing human gastric carcinoma SGC 7901 was investigated. Four to five molecules of DM were introduced into each molecule of antibody while the antigen - binding capacity of the antibody retained well. This conjugate cdud satisfactorily localize in the tumor tissue. Nlnty six hours after intraperitoneal injection of 125I MGb2- DM conjugate, the ratio of radioactivity per milligram of tumor to that per milligram of blood reached 250, the result being quite similar to that of unmodified MGb2.
出处
《第四军医大学学报》
1990年第6期430-432,共3页
Journal of the Fourth Military Medical University
关键词
胃肿瘤
碘放射同位素
单克隆抗体
antibodies, monodonal
daunomycin
antibody toxin conjugates
stomach neoplasm, iodine radioisotope
mice, nude
