摘要
目的:利用生物芯片技术分析人参对衰老小鼠脑组织基因表达谱的影响,探讨人参作用机理研究方法.方法:20只雌性小鼠用D-半乳糖诱导为亚急性衰老模型小鼠,并随机分为两组:实验组和对照组.实验组小鼠每日给服人参水煎剂,对照组小鼠每日给服等量生理盐水作为空白对照.从小鼠脑组织中抽提mRNA,用Cy3和Cy5两种不同的荧光染料,通过逆转录反应,将实验组和对照组小鼠的脑组织细胞mRNA分别标记成两种探针,等量混合后与小鼠基因表达谱芯片杂交,并对Cy3、Cy5荧光信号做扫描分析.结果:芯片扫描显示,实验组和对照组之间显著差异表达的基因有13条,且均为表达下调基因.结论:人参对衰老小鼠脑组织的基因表达具有显著影响,Nckap1基因及Atp5a1基因可能是人参抗衰老作用的靶基因.
Objective： To investigate the molecular mechanism of Panax. Methods： The subacute aging model mice induced by D-galactose were applied. Equal quantity mRNA derived from the experimental group and the control group were labeled with cy3-dUTP and cy5-dUTP as probe respectively. The mixed probes were then hybridized with BiostarM-20s cDMA mieroarray. The acquired image was analyzed by GenePix Pro3.0. Results： 13 differentially expressed genes were screened out. They are all protein encoded genes. Conclusion： It＇s proved that Panax indeed have effects on gene expression profile of aging model mice brain tissue. Nckapl and AtpSal gene may be the target genes of the anti-aging effect of Panax.
出处
《传感技术学报》
CAS
CSCD
北大核心
2006年第05B期2122-2125,共4页
Chinese Journal of Sensors and Actuators
基金
西北工业大学青年科技创新基金资助(M016214)
