摘要
目的了解2006年春季江苏省一起流感疫情的流行病学和毒株的变异情况。方法对一起流感暴发疫情进行流行病学调查,使用荧光定量RT-PCR快速检测病原体,采用MDCK细胞分离流感病毒,用血凝抑制实验对病毒株分型鉴定,对该起疫情的流感毒株进行种系发生分析,并与同一时间的其他地区乙型流感毒株的HAl基因序列进行比较。结果此起疫情发病人数为357人,发病率为13.2%,23份咽拭子标本中分离出16份毒株,病原为B型Victoria株型流感病毒。基因种系发生树分析证明了它们的HAl基因不同于B/HongKong/330/2001和B/Zhejiang/2/2001病毒。结论2006年春季江苏省人群中流行的乙型流感病毒维多利亚毒株系已发生变异,因此加强流感病毒病原学监测,对于防止流感疫情暴发具有重要意义。
Objective To study the epidemiological trend of a influenza outbreak and the antigenic drift of influenza B since 2006 in Jiangsu province. Methods Epidemiological investigation and sample collection were applied. Influenza virus was detected by real-time RT-PCR immediately and isolated by MDCK cells. The virus strains were identified by hemagglutination inhibition（HI）. The sequence of HAl gene of influenza B viruses isolated from various areas of Jiangsu province was analyzed by phlogenetic tree. Results 357 cases were reported in this influenza outbreak, the attack rate was 13. 2%, 16 influenza B virus strains were isolated from 23 throat swabs. The HA1 gene of these isolates was different from B/Hong Kong/330/2001 and B/Zhejiang/2/2001. Conclusion The discovery of the varied victoria like strain of influenza B virus in Jiangsu province demands the enhancement of influenza surveillance.
出处
《公共卫生与预防医学》
2006年第5期29-31,34,共4页
Journal of Public Health and Preventive Medicine
关键词
乙型流感
暴发
流行病学
血凝素
序列分析
Influenza B virus
Outbreak
Epidemiology
Hemagglutination
Sequence analysis
