摘要
A novel fimctional gene P12 was isolated from neural stem cells cultured in differentiation medium. The fully length cDNA of P12 gene was cloned and sequenced. Result showed that it contains an open reading frame encoding a protein of 291 amino acids. Further, this gene was transferred into neural stem cell. Functional analysis suggests that the expression of p12 protein is closely correlated with differentiation of neural dendrite configuration. In addition, to obtain encoding protein, P12 sequence was also expressed in Pichia pastoris yeast.
A novel fimctional gene P12 was isolated from neural stem cells cultured in differentiation medium. The fully length cDNA of P12 gene was cloned and sequenced. Result showed that it contains an open reading frame encoding a protein of 291 amino acids. Further, this gene was transferred into neural stem cell. Functional analysis suggests that the expression of p12 protein is closely correlated with differentiation of neural dendrite configuration. In addition, to obtain encoding protein, P12 sequence was also expressed in Pichia pastoris yeast.
基金
Project supported by National Basic Research Program of China(Grant No . 973 -2006CB500702) , National Natural Science Foundation of China (Grant No .30570590) ,Science Foundation of Shanghai Municipal Commission of Science and Technology(Grant Nos . 03JC1403 , 03JC14061) , and Scientific Research Foundation of the Returned Overseas Chinese Scholars , State Education Ministry (Grant No .JWSL2003406)
