摘要
取昆明种新生小鼠后肢肌肉1 mm^3置于普通培养皿内,在含羊成骨蛋白(sBMP)1、5、10、50 μg/ml的1640培养基内培养10 d,sBMP各浓度组均有成软骨反应,10μg/ml组成骨反应最好,成骨率达80％,有4个肌肉块完全转化成软骨团。本实验建立了一个体外诱导成骨的实验模型,为骨诱导的深入研究提供了一个简便的组织培养方法。
Sheep bone morphogenetic protein (sBMP) was chemically extracted from sheep bone matrix. When exogenous sheep BMP was introduced into 1640 culture medium at the concentrations of 1-50 μg/ml, new cartilage developed inside the rat muscle during a 10-day latent period. With a mean rate of 52% of chondrogenesis new cartilage tissues developed in 80% of explants in the medium containing 10 μg/ml of sBMP. The new method established in this experiment provides a useful model of tissue culture for making a progressive researching of bone inducing.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1990年第6期511-514,共4页
Academic Journal of Second Military Medical University
关键词
成骨蛋白
诱导成骨
组织培养
bone morphogenetic protein
tissue culture
bone inducing
