摘要
目的 建立紫玉盘总黄酮的含量测定方法并检测其不同部位总黄酮的含量。方法 以芦丁为对照品，采用分光光度法于500nm处测定艾纳香中总黄酮的含量。结果芦丁在0—0．076mg／ml（r=0．9995）范围内线性关系良好，平均回收率为99．7％，RSD=2．3％（n=5），其叶、枝条、根含总黄酮分别为10．53％，5．60％，3．87％。结论 该方法简便、准确、可靠，可作为该药材中总黄酮含量检测的方法。
Objective To develop a quantitative method for the determination of total flavonoid in different sections in Uvaria microcarpa. Methods Using rutin as a reference substance, the total flavonoids in Uvaria microcarpa was determined by UV absorption at 500 nm. Results The linearity of mtin was obtained within the range of 0 to 0.076 mg/ml（r =0.999 5）. The average recovery was 99.7% and RSD was 2.3%. The content of total flavonoid in Uvaria microcarpa from leaf was 10.53% ,branch was 5.60%, root was 3.87% （n =5）. Conclusion The method is convenient and reliable. It can be used as a quality control method to determine total flavonoids of Uvaria microcarpa.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第10期1900-1901,共2页
Lishizhen Medicine and Materia Medica Research
基金
广西自然科学基金资助项目(No.0342002-3)
关键词
紫玉盘
总黄酮
分光光度法
Uvaria microcarpa
Total flavonoid
Determination
