摘要
目的：研究金属离子与细胞膜的相互作用与金属离子性质的关系。方法：用马来酰亚胺（ＭＳＬ）标记ＥＳＲ及Ｎ－［３－芘］－马来酰胺和荧光胺荧光标记方法研究了Ｃｄ（２＋）、Ｎｉ（２＋）、Ｃｕ（２＋）、Ｐｂ（２＋）、Ｚｎ（２＋）、Ｃｏ（２＋）、Ｍｎ（２＋）、Ｃａ（２＋）、Ｃｅ（３＋）９种金属离子与人红细胞膜的作用。结果：实验表明这些金属离子与膜的作用与Ｌｅｗｉｓ酸的软硬度有关，但Ｐｂ（２＋）与膜作用的波谱学行为象Ｃａ（２＋）；Ｃｅ（３＋）的行为也与Ｃａ（２＋）相同，而Ｍｎ（２＋）引起了膜Ｗ／Ｓ比值明显下降，又与Ｃｕ（２＋）相似。结论：金属离子与膜的作用方式与其化学性质有关。
Objective:The present study deals with the interaction of metal ions with cell membrane and its relation to properties of metal ions. MethOds:By maleimide spin labeling ESR and N-[3-P]-M or fluorescamine labeled fluorometric method ,the interaction of metal ions Cd￣(2+),Ni￣(2+),Cu￣(2+),Pb￣(2+),Zn￣(2+),Co￣(2+),Mn￣(2+),Ca￣(2+),Ce￣(3+) with human erythrocyte membranes was studied.Results:The experiments in-dicated that the interaction between these metal ions and membranes was relative to hardness-softness of Lewis acid of the metal ions significantly.But Pb￣(2+) behaves like Ca￣(2+) on membranes as shown by spectroscopy,Ce￣(3+) behaves the same as Ca￣(2+); Mn￣(2+) induced considerable decrease of W/S value of la-beled membranes,somewhat like Cu￣(2+).Conclusion:The interaction of metal ions with human erythro-cyte membranes is ralative to the chemical properties of metal ions.
出处
《北京医科大学学报》
CSCD
1996年第6期469-471,共3页
Journal of Peking University(Health Sciences)
基金
国家自然科学基金
关键词
金属
红细胞膜
荧光光度法
Metal
Erythrocyte membrane
Maleimides
Fluorophotmetry
