摘要
本实验利用饱和硫酸铵盐析和葡聚糖凝胶过滤层析,从猪视网膜中提取了S抗原。50 μg S抗原伴等量完全佛氏佐剂,注射于豚鼠后足掌肉垫皮下。两周后,诱发了中度至严重的葡萄膜视网膜炎。其病理特征是:脉络膜内有大量淋巴细胞浸润;由前向后,逐渐增厚。炎症变化与病程有关。超微结构特征性改变是视网膜感光细胞内、外节水肿,变性,坏死。提示:S抗原有强烈诱发自身免疫性葡萄膜视网膜炎的作用;外层网膜是较敏感的靶器官。
The soluble antigen was isolated from pig retinal extract by precipitation with ammonium sulfate followed by molecular sieve. 50 μg S antigen with equal volume complete Freund's adjuvant was injected into double hind foodpads of guinea pigs. Two weeks later, experimental autoimmune uveitis developed. Its pathologic feature is that a great number of lymphocytes infiltrated into chroid which was thickened gradually from anterior to posterior. Inflammatory variation was relative to disease phase. Ultrastructure pathology was characterized by swelling and necrosis in the inner and outer segments of photorecepter cells. This result suggests that S antigen has significant effect on inducing experimental autoimmune uveitis and the outer layer of retina is the sensitive target organ of S antigen.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1990年第5期428-432,共5页
Academic Journal of Second Military Medical University
关键词
S抗原
葡萄膜炎
光镜
电镜
S antigen
immunologic diseases
uveitis
diseae models, animal
