摘要
使用特异荧光探针定位术，对不同培养时期人喉癌细胞系（ＨＥｐ２）ＭＴＳ表层细胞的Ｆ－肌动蛋白微丝骨架进行了对比观察。结果表明，随着多细胞肿瘤球体（ＭＴＳ）转移培养时间的延长，表层细胞中出现数目较多的Ｆ－肌动蛋白小体，其直径约为０．３～１．０μｍ。Ｆ－肌动蛋白微丝结构的改变，可能与ＭＴＳ表层细胞的侵袭与转移活性增强有关，但也有可能是表层细胞在不同生理状况下的一种反应。
We have compared the cytoskeleton of F-actin microfilaments in the outer cells of multicellular tumor spheroids(HEp 2) with different culture duration by staining with TRITC labeled phalloidin. Results showed that many F-actin aggregates appeared in the outer cells with the prolongation of MTS culture. These aggregates are dot-like with diameter 0. 3～1. 0 μm. The reorganization of F-actin microfilaments may not only be related to the greater potential capacity of outer cells to invade and metastasis ,but may also reflect the physical condition of the outer cells.
出处
《首都医科大学学报》
CAS
1996年第4期253-254,共2页
Journal of Capital Medical University
关键词
表层细胞
肌动蛋白
肿瘤培养
HEp 2
outer cells of MTS
F-actin
TRITC labeled phalloidin
