摘要
采用细胞融合技术制备抗人小扁豆凝集素结合型甲胎蛋白异质体单克隆抗体,建立单克隆抗体“双位点夹心”ELISA血清学检测方法。通过对69例原发性肝细胞癌、67例良性肝病、30例妊娠妇女和30例健康成人血清检测结果分析,表明该方法操作简便,结果准确,重复性好,不仅假阳性率低,并适用于低浓度甲胎蛋白原发性肝细胞癌的早期诊断,使良、恶性肝病的鉴别诊断率明显提高,具有较高的临床实用价值。
In this paper, we report the study concerning the preparation and application of anti-human heterogeneous AFP-R-LCA monoclonal antibodies (VG5 VD12 VB5 VA8 VD12). These McAbs had higher affinity and specificity to AFP-R-LCA than the routinely used anti-AFP polyclonal antibody. Competitive assays indicated that they could bind to new antigenic determinants which are different from the previously reported a and b sites. In an assay of the McAbs, we first set up a two site sandwich ELISA method and then tested, using this method, serum samples from 69 patients with PHC, 67 patients with benign liver diseases, 30 pregnant women and 30 normal controls. The results showed that this two site sandwich ELISA method had low pseudo-positive rate. The detection range was 5 1000 ng/ml, with the properties of simplicity, accuracy and reproducibility. It could be used not only for the early diagnosis of PHC in patients with AFP levels less than 400 ng/ml, but also for the differential diagnosis of PHC from benign liver diseases.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1990年第3期201-205,共5页
Academic Journal of Second Military Medical University
关键词
甲胎蛋白
单克隆抗体
肝细胞癌
凝集素
Lens culinaris agglutimn
AFP
monoclonal antibody
heterogenecity
