摘要
本文报道感染伯氏疟原虫小鼠血清在琼脂糖凝胶上,单独扩散可形成白色沉淀环的现象。感染伯氏疟原虫ANKA株小鼠血清组及感染该株抗氯喹系小鼠血清组的沉淀环阳性率分别为73.7％(28/38)和86.5％(32/37),其沉淀环的清晰度和致密度在感染疟原虫后第2～6d有逐渐增强的趋势。尢疟原虫感染的正常小鼠血清对照组阳性率仅为25.6％(10/39),其沉淀环均不清晰,也不随时间推移而变化。实验组与对照组沉淀环阳性率比较,相差非常显著(P<0.01)。此现象为研究疟疾早期血清学诊断提供了新的线索。
A new phenomenon of ring-like precipitum formed by diffusing sera of mice infected with Plasmodium berghei in agarose gel is reported. When sera of mice infected with either P. berghei ANKA strain or the strain's chloroquine-resistant line were poured into wells of agarose gel for diffusion at 37℃ for 8 to 12 h, a ring-like precipitum around the wells appeared. The optimal time for detecting the precipitum was the second to sixth day following inoculation of mice with malaria parasites. The optimal RBC infection rate of mice was 3% to 10%. Higher infection rate ( >20%) or diffusion conducted after the sixth day of the inoculation was inappropriate to the appearance of the precipitum. The positive rates of the precipitum in the group of serum of mice infected with P. berghei ANKA strain, and the group of serum of mice infected with the strain's chloroquine-resistant line were 73. 7% (28/39) and 86. 5% (32/37), respectively. The clearness and denseness of the precipitum in the two groups were increased during the period of 2nd to 6th day after infection of mice with malaria parasites. The positive rate of the control group (serum of normal mice) was 25. 6% (10/39), and the precipitum formed in the group was obscure (±) and remained the same from 2nd to 8th day. There was significant difference in positive rates between the experimental group and the control ( P<0. 01). The phenomenon found in this study could provide a new clue to develop a new type serological method for early detection of malarial infection.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1990年第2期107-109,共3页
Academic Journal of Second Military Medical University
关键词
疟疾
伯氏疟原虫
血清学
诊断
malaria
Plasmodium berghei
serological diagnosis
