摘要
采用高效液相色谱法测定紫杉醇微乳型注射剂中紫杉醇的含量。色谱柱为Phenomenex ODS3，流动相为甲醇-乙腈-水（40：30：30），流速为1ml·min^-1，柱温为40℃，检测波长为227nm，内标物为炔诺酮。在100-1000μg·ml^-1浓度范围内，紫杉醇浓度与紫杉醇／炔诺酮峰面积比之间具有良好的线性关系，r=0．9999。加样回收率为99．77％，RSD为0．54％。本方法可靠，准确。
A HPLC method was established for the determination of paclitaxel in the microemulsion injection. The separation was performed using a Phenomenex ODS3 column, and a mobile phase of methanol, acetonitril and water （40 ： 30 ： 30） with a flow rate of lml · min^-1. The detective wavelength was at 227nm. Anorethisterone was used as internal standard. The calibration curve was in the range of 100-1000μg （r=0. 9999）. The average recovery was 99.77% （RSD=O. 54%）. The method is accurate and reliable.
出处
《中国药事》
CAS
2006年第10期610-611,620,共3页
Chinese Pharmaceutical Affairs
