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结核杆菌抗原诱导的人γδT细胞表达L-选择素的调控机制 被引量：1

The modulatory mechanism on the L-selectin expression from activated human γδT cells induced by Mycobacterium tuberculosis peptide antigen
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摘要 采用CD62L生物素-链霉亲和素染色流式细胞术检测L-选择素在细胞表面表达的方法，分别观察新鲜PBMC和刺激后培养3～21d的γδT细胞表面L-选择素的表达，并研究培养6～7d的MtbAT预先用LY294002（PI3K途径抑制剂）、SB203580（p38途径抑制剂）、PD98059（MEK抑制剂）分别处理20min，然后用Mtb-Ag再刺激3h时γδT细胞表面L-选择素的表达情况。结果显示新鲜PBMC中γδT细胞L-选择素的表达率为（43．9％±6．7％），γδT细胞L-选择素的表达率为（76．6％±7．9％），有显著差异（P〈0．05）。用Mtb-Ag激活后培养第6天时L-选择素在γδT细胞的表达达到高峰（90．7％）；以后L-选择素表达逐渐下降，15～21dγδT细胞L-选择素的表达在低水平（16．2％～10．6％）维持；Mtb—Ag再刺激MtbAT诱导γδT细胞L-选择素表达下调为69．5％，预先加入LY294002或PD98059，则L-选择素表达率分别为83．1％和78．7％；而加入SB203580未见明显影响。提示γδT细胞活化时L-选择素表达调控与PI3K、Ras／Erk途径有关，但与p38MAPK途径无关。 The expression of L-selectin on the surface of γδT cells induced by Mycobacterium tuberculosis peptide antigen （Mtb- Ag） in fresh PBMC cultures cultivated for 3-21 days was detected by means of the flow cytometry （FCM） with the CD62L-biotin-streptavidin RED-670 staining, in which the Mtb-Ag-induced cell cultures cultivated for 6-7 days were pre-treated with SB203580 （an inhibitor for p38 pathway）, LY294002 （an inhibitor for PI3K pathway） or PD980590 （an hihibitor for MEK） for 20 minutes and then restimulated with Mtb-Ag for 3 hours. It was demonstrated that the percentage of L-selectin from γδT cells in the fresh PBMC was significantly lower than that of αβ T cells （43. 9% versus 76. 6%）. The expression of CD62L reached its climax on day 6 of γδT cell cultures （90. 7%）, then decreased and maintained at lower level . Meanwhile, the expression of L-selectin from γδT ceils maintained at lower level from 15-21 days （16.2%-10.6%）, but it was down-regulated to 69.5%, when the Mtb-Ag induced γδT cells was restimulated with Mtb-Ag. In addition, pretreatment of the Mtb-Ag induced ceils with LY294002 or PD98059 could induce the appearance of the L-selectin expression rates of 83.1% and 78.7 respectively, but no any influence could be observed when pretreated with SB203580. These results suggest that the signal pathways including PI3K and Ras/Erk, but not p38 MAPK, participate in the L-selectin expression from activated human γδT cells restimulated by Mycobacterium tuberculosis peptide antigen.
作者 武文娟 李柏青
机构地区 蚌埠医学院生物化学与分子生物学教研室 安徽省感染与免疫重点实验室(蚌埠医学院)
出处 《现代免疫学》 CAS CSCD 北大核心 2006年第5期413-417,共5页 Current Immunology
基金 国家自然科学基金资助项目(3007021) 安徽省高校省级重点科研资助项目(2004sys008) 蚌埠医学院科研基金资助项目(BY0508)
关键词 L-选择素 ΓΔT细胞 结核杆菌抗原 信号转导 L-selectin γδT lymphocytes Mycobacterium tuberculosis antigen signal transduction
分类号 R329.25 [医药卫生—人体解剖和组织胚胎学]
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参考文献21

  • 1Picker LJ,Butcher EC.Physiological and molecular mechanisms of lymphocyte homing[J].Annu Rev Immunol,1992,10:561-591.
  • 2Smith JA.Neutrophils,host defence and inflammation:a double-edged sword[J].J Leukocyte Biol,1994,56(6):672-686.
  • 3Chao CC,Jensen R,Dailey MO.Mechanisms of L-selectin regulation by activated T cells[J].J Immunol,1997,159(4):1686-1694.
  • 4Spertini O,Freedman AS,Belvin MP,et al.Regulation of leukocyte adhesion molecule-1(TQ1,Leu-8) expression and shedding by normal and malignant cells[J].Leukemia,1991,5(4):300-308.
  • 5Junger M,Friedrich B,Hahn J,et al,Down regulated L-selectin expression on lymphocytes in patients with chronic venous infection[J].Clin Hemorheol Nucricur,2001,25(1):21-30.
  • 6Chiro C,Arabito E,Landi F,et al.Analysis of circulating γδT cells in children affected by IgE-associated and non-IgE-associated allergic atopic eczema/dermatitis syndrome[J].Clin Exp Immunol,2005,141(1):116-121.
  • 7van Wely CA,Beverley PCL,Brett SJ,et al.Expression of L-selectin on Th1 cells is regulated by IL-12[J].J Immunol,1999,163(3):1214-1221.
  • 8Gerli R,Gresele P,Bistoni O,et al.Salicylates inhibit T cell adhesion on endothelium under nonstatic conditions:induction of L-selectin shedding by a tyrosine kinase dependent mechanism[J].J Immunol,2001,166(3):832-840.
  • 9Kaldjian EP,Stodman LM.Regulation of L-selectin mRNA in Jurkat cells[J].J Immunol,1995,154(4):4351-4362.
  • 10Karin K,Jens D,Eva CM,et al,The interaction of protein kinase C isozymes α,ι,θ with the cytoplasmic domain of L-selectin is modulated by phosphorylation of the receptor[J].J Biol Chem,2004,279(33):34472-34480.

二级参考文献18

  • 1梅传忠,沈继龙,陈勇,李柏青.乙醇及二甲基亚砜对人γδT细胞和αβT细胞凋亡与细胞活性的影响[J].安徽医科大学学报,2004,39(3):168-173. 被引量：4
  • 2Chao CC, Jensen R, Dailey MO. Mechanisms of L-selectin regulation by activated T cells[J]. J Immunol, 1997,159 (4):1 686～1 694.
  • 3Picker LJ, Martin RJ, Trumble A, et al. Differential expression of lymphocyte homing receptors by human menory/effector T cells in pumonary versus cutaneous immue effector sites [ J ]. E J Immunol, 1994,24 (6): 1 269～1 277.
  • 4Boom WH,Balaji KN, Nayak R, et al. Characterization of a 10- to 14-kilodalton protease-sensitive Mycobacterium tuberculosis H37Ra antigen that stimulates human γδT cells [ J ]. Infect Immun ,1994,62 (12):5 511～5 518.
  • 5van Wely CA, Beverley PC, Brett SJ, et al. Expression of Lselectin on Th1 cells is regulated by IL-12[J]. J Immunol, 1999,163 (3):1 214～1 221.
  • 6Laurent M,Pado TB. T lymphocyte activation intiates the degradation of the CD62L encoding mRNA and increase the transcription of the corresponding gene[J]. Immunol Lett ,2004,94 (1-2) :115～122.
  • 7Borges E, Tietz W, Steegmaier W, et al. P-selectin glyc oprotein ligand-1(PSGL-1)on T helper 1 but not on T helper 2 cells binds to P-selectin and supports migration into inflamed skin[J]. J Exp Med, 1997,185 (3) :573～578.
  • 8Boom WH, Balaji KN, Nayak R, et al. Characterization of a 10-to 14-kilodalton protease-sensitive Mycobacterium tuberculosis H37Ra antigen that stimulates human γδT cells[J]. Infect Immun,1994,62(12):5111-5118.
  • 9Li BQ, Rossman MD, Imir T, et al. Disease-specific changes in γδT cell repertoire and function in patients with pulmonary tuberculosis[J]. J Immunol, 1996,157(9):4222-4235.
  • 10Li BQ, Bassiri H, Rossman M, et al. Involvement of the Fas/Fas ligand pathway in activation-induced cell death of mycobacteriareactive human γδT cells: a mechanism for the loss of γδT cells in patient with pulmonary tuberculosis[J]. J Inmaunol, 1998,161:1558-1567.

共引文献25

同被引文献14

  • 1陈勇,吕合作,李柏青,王伟,张海峰.纯化的结核杆菌多肽抗原刺激人γδT细胞的效应分析[J].中国免疫学杂志,2004,20(10):661-664. 被引量：9
  • 2武文娟,沈继龙,李柏青.MtbAg体外激活扩增的人γδT细胞表面L-选择素的动态变化[J].蚌埠医学院学报,2005,30(3):192-194. 被引量：3
  • 3Picker LJ, Butcher EC. Physiological and molecular mechanisms of lymphocyte homing. Annu Rev Immunol, 1992, 10: 561-591.
  • 4Smith JA. Neutrophils, host defense, and inflammation: a double-edged sword. J Leukocyte Biol, 1994, 56(6) : 672-686.
  • 5Chao CC, Jensen R, Dailey MO. Mechanisms of L-selectin regulation by activated T cells. J Immunol, 1997, 159 (4) : 1686- 1694.
  • 6Richards H, Longhi MP, Wright K, et al. CD62L (L-selectin) down-regulation does not affect memory T cell distribution but failure to shed compromises anti-viral immunity. J Immunol, 2008, 180( 1 ) : 198-206.
  • 7Lee D, Schultz JB, Knauf PA, et al. Mechanical shedding of L- selectin from the neutrophil surface during rolling on sialyl Lewis x under flow. J Biol Chem, 2007, 282(7) : 4812-4820.
  • 8Gerli R, Gresele P, Bistoni O, et al. Salicylates inhibit T cell adhesion on endothelium under nonstatic conditions: induction of L-selectin shedding by a tyrosine kinase dependent mechanism. J Immunol, 2001, 166(3): 832-840.
  • 9Kaldjian EP, Stodman LM. Regulation of L-selectin mRNA in Jurkat cells. J Immunol, 1995, 154(4) : 4351-4362.
  • 10Karin K, Jens D, Eva CM, et al. The interaction of protein kinase C isozymes α,ι,θ with the cytoplasmic domain of L-selectin is modulated by phosphorylation of the receptor. J Biol Chem, 2004, 279(33): 34472-34480.

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现代免疫学
2006年 第5期

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