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基质溶解素及其反义寡核苷酸对肺腺癌A549细胞增殖的作用 被引量：1

Effect of matrilysin and its antisense oligonucleotide on proliferation of lung adenocarcinoma cell line A549
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摘要 目的探讨重组基质溶解素(recombinantmatrilysin,rMMP-7)和脂质体-MMP-7反义寡核苷酸(antisenseoli-gonucleotideofmatrilysinbyliposometransfection,LIPO-MAON)对肺腺癌A549细胞和人脐静脉血管内皮细胞(humanum-bilicalveinendothelialcell,HUVEC)增殖的影响。方法①构建MMP-7的反义寡核苷酸。②MTT法检测rMMP-7和LI-PO-MOAN作用下A549和HUVEC的增殖状况。③RT-PCR法检测rMMP-7和LIPO-MAON作用下腺癌A549细胞和HU-VECMMP-7mRNA的表达。结果0.75、1.0μg/mlrMMP-7对HUVEC增殖有促进作用,各种浓度的LIPO-MAON无作用;对A549细胞的增殖,0.5、0.75、1.0μg/ml的rMMP-7均有促进作用;而高浓度(7.5、10nmol/ml)的LIPO-MAON对A549细胞增殖有明显抑制作用。RT-PCR检测结果表明,HUVEC不表达MMP-7mRNA;rMMP-7能增加A549细胞MMP-7mRNA的表达,LIPO-MAON可降低其MMP-7mRNA的表达(P<0.05)。结论在本试验浓度范围内高浓度的MMP-7能促进正常血管内皮细胞和肺腺癌细胞的增殖,其反义寡核苷酸不能抑制正常血管内皮细胞增殖,但高浓度的反义寡核苷酸能抑制肺腺癌细胞的增殖。 Objective To study the effect of the recombinant matrilysin （rMMP-7） and its antisense oligonucleotide transfected by liposome （LIPO-MAON） on the proliferation of lung adenocarcinoma cell line A549 and human umbilical vein endothelial cells （HUVEC）. Methods Antisense oligonucleotide of matrilysin was constructed by liposome transfection. The proliferation of HUVEC and A549 was detected by MTT assay in case of rMMP-7 or LIPO-MAON existence. The expression of MMP-7 mRNA in both HUVEC and A549 was examined by semi-quantitative RT-PCR assay. Results The proliferation of both HUVEC and A549 cell line was accelerated by high level of rMMP-7 （ 0.75, 1.0 μg/ml） , and the inhibited proliferation was only found in A549 cell line treated with high concentration of LIPO-MAON （7.5, 10 nmol/ml）, but not in HUVEC. By RT-PCR assay, no expression of MMP-7 mRNA was found in HUVEC; however, enhanced or decreased expression of mRNA were found when A549 cell line was treated respectively with rMMP-7 or LIPO-MAON （ P 〈 0. 05）. Conclusion The proliferation of both HUVEC and A549 cell line can be accelerated by high level of rMMP-7. No effect of matrilysin antisense oligonucleotide on the proliferation of HUVEC is found, while at high concentration it can inhibit the proliferation of A549 cell line.
作者 王志新 徐飞 谢启超 陈正堂
机构地区 第三军医大学新桥医院肿瘤科
出处 《第三军医大学学报》 CAS CSCD 北大核心 2006年第20期2044-2046,共3页 Journal of Third Military Medical University
关键词 基质溶解 MMP-7反义寡核苷酸 肺腺癌细胞A549 人脐静脉血管内皮细胞 matrilysin, MMP-7-antisense oligonucleotide, human umbilical vein endothelial cell, lungadenocarcinoma cell line A549
分类号 R329.28 [医药卫生—人体解剖和组织胚胎学] R730.23 [医药卫生—肿瘤]
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参考文献11

  • 1李向东,鲁开化,郭树忠,高政慧.血管内皮细胞培养与观察[J].中国美容医学,2001,10(3):189-191. 被引量：6
  • 2ISHII Y,NAKASATO Y,KOBAYASHI S,et al.A study on angiogenesis-related matrix metalloproteinase networks in primary hepatocellular carcinoma[J].J Exp Clin Cancer Res,2003,22(3):461-470.
  • 3IMPOLA U,UITTO V J,HIETANEN J,et al.Differential expression of matrilysin-1 (MMP-7),92 kD gelatinase (MMP-9),and metalloelastase (MMP-12) in oral verrucous and squamous cell cancer[J].J Pathol,2004,202(1):14-22.
  • 4WATANABE H,IWASE M,OHASHI M,et al.Role of interleukin-8 secreted from human oral squamous cell carcinoma cell lines[J].Oral Oncol,2002,38(7):670-679.
  • 5LIU X P,KAWAUCHI S,OGA A,et al.Prognostic significance of matrix metalloproteinase-7 (MMP-7) expression at the invasive front in gastric carcinoma[J].Jpn J Cancer Res,2002,93(3):291-295.
  • 6WILSON C L,HEPPNER K J,LABOSKY P A,et al.Intestinal tumorigenesis is suppressed in mice lacking the metalloproteinase matrilysin[J].Proc Natl Acad Sci U S A,1997,94(4):1402-1407.
  • 7FINGLETON B,VARGO-GOGOLA T,CRAWFORD H C,et al.Matrilysin[MMP-7] expression selects for cells with reduced sensitivity to apoptosis[J].Neoplasia,2001,3(6):459-468.
  • 8MITSIADES N,YU W H,POULAKI V,et al.Matrix metalloproteinase-7-mediated cleavage of Fas ligand protects tumor cells from chemotherapeutic drug cytotoxicity[J].Cancer Res,2001,61 (2):577-581.
  • 9HASHIMOTO G,INOKI I,FUJII Y,et al.Matrix metalloproteinases cleave connective tissue growth factor and reactivate angiogenic activity of vascular endothelial growth factor 165[J].J Biol Chem,2002,277(39):36288-36295.
  • 10YONEMURA Y,ENDO Y,FUJITA H,et al.Inhibition of peritoneal dissemination in human gastric cancer by MMP-7-specific antisense oligonucleotide[J].J Exp Clin Cancer Res,2001,20(2):205-212.

二级参考文献12

  • 1何红兵,潘玉先,刘兰平,杨继震.成人静脉内皮细胞单次传代大规模体外培养[J].第一军医大学学报,1995,15(3):198-201. 被引量：9
  • 2Salmela MT,Karjalainen LM,Puolakkainen P,et al.Upregulation and differential expression of matrilysin(MMP-7) and metalloelastase(MMP-12) and their inhibitors TIMP-1 and TIMP-3 in Barrett′s oesophageal adenocarcinoma[J].Br J Cancer,2001,85(3): 383
  • 3Zengzhaoshi,Shuweiping,Cohen Alfred M.Matrix metalloproteinase-7 expression in colorectal cancer liver metastases: evidence for involvement of MMP-7 activation in human cancer metastases[J].Clin Cancer Res,2002,8(1): 144
  • 4Gakuji H,Isao I,Yutaka Fi,et al.Matrix metalloproteinases cleave connective tissue growth factor and reactivate angiogenic activity of vascular endothelial growth factor 165 J[J].Biol Chem,2002,277(39): 36288
  • 5Ougolkov AV,Yamashita K.Oncogenic beta-catenin and MMP-7(matrilysin) cosegregate in late-stage clinical colon cancer[J].Gastroenterology,2002,122(1): 60
  • 6Nishizuka I,Ichikawa Y,Ishkawa T,et al.Matrilysin stimulates DNA synthesis of cultured vascular endothelial cells and induces angiogenesis in vivo[J].Cancer letter,2001,173(2):175
  • 7Asaki T,Matsuoka H,Sugiyama M,et al.Matrilysin(MMP-7) as a significant determinant of malignant potential of early invasive colorectal carcinomas[J].Br J Cancer,2001,84(10): 1317
  • 8Masaki T,SM,Matsuoka H.Matrix metalloproteinases may contribute compensationally to tumor invasion in T1 colorectal carcinomas[J].Anticancer Res,2003,23(5b): 4169
  • 9Fromowitz FB,Viola WV,Chao S,et al.Ras p21 expression in the progression of breast cancer[J].Human Pathol,1987,18(12):1268
  • 10Weidner N.Intratumor microvessel density as a prognostic factor in cancer[J].Am J Pathol,1995,147(1):9

共引文献11

同被引文献12

  • 1Mc Cawley LJ, Matrisian LM. Matrix metalloproteinases : multifunctional contributors to tumor progression [J]. Mol Med Today,2000,6(4) : 149-156.
  • 2Shigemasa K, Tanimoto H, Sakata K, et al. Induction of matrix metalloproteinase 7 is common in mucinous ovarian tumors including early stage disease[J]. Med Oncol,2000, 17(1 ) : 52- 58.
  • 3Nishizuka I, Ichikawa Y, Ishkawa T,et al. Matrilysin stimulates DNA synthesis of cultured vascular endothelial cells and induces angiogenesis in vivo [J]. Cancer letter,2001,173(2) : 175.
  • 4Wilson CL, Heppner KJ,Labosky PA, et al. Intestinal tumorigenesis is suppressed in mice lacking themetallop roteinasematrily sin[J]. Proc Nail Acad Sci USA, 1997, 94 (4) : 1402- 1407.
  • 5Fingleton B, Vargola T, Crawford HC, et al. Matrilysin [MMP-7] expression selects for cells with reduced sensitivity to apoptosis[J]. Neop lasia, 2001,3(6): 459-468.
  • 6Mitsiadesn, Yuw H, Poulaki V, et al. Matrixmetalloproteinase mediated cleavage of Fas ligand protects tumor cells from chemotherapeutic drug cytotoxicily[J]. Cancer Res,2001, 61(2):577-581.
  • 7Yamamoto, Azumano I, Mai M, et al. Expression and tissue localization of matrix metalloproteinases 7 (matrilysin) in human gastric carcinomas[J]. Int J Cancer, 1998, 79(6):187- 194.
  • 8Wysk M, Yang DD, Lu HT, et al. Requirement of mitogenactivated protein kinase kinase 3 (MKK3) for tumor necrosis factor induced cytokine expression [J]. Proc Natl Acad Sci USA,1999,96(7) :3763-3768.
  • 9Nebreda AR, Porras A. p38 MAP kinases: beyond the stress response[J]. Trends Biochemeri, 2000,25(6) : 257-260.
  • 10Scbmalfeldt B, Prechtel D, Hatting K, et al. Increased expression of matrix metalloporteinase-s (MMP-2, MMP-9), and the urokinase lype plasminogen activator is associated with progression from benign to advanced ovarian cancer[J]. Clin Cancer Res,2001,7(8) :2396-2404.

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第三军医大学学报
2006年 第20期

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