摘要
目的检测和分析转移特性不同的两个小鼠肝癌细胞亚系线粒体DNA(mtDNA)的遗传变异,探讨线粒体DNA遗传改变与肿瘤发生发展的关系。方法PCR-RFLP和序列测定技术。结果对mtDNA的tRNAIle+GlN+Met基因和ND3基因以及D-loop片段进行的扩增和限制性片段长度多态性分析结果显示,无扩增片段长度呈多态性,且这两个肝癌细胞系mtDNA的所有限制性片段方式和大小完全一致。序列测定发现,这两个肝癌细胞系在线粒体DNA的D-loop区存在序列差异。结论mtDNA非编码区内的遗传改变,反映了肿瘤发生发展过程中环境和遗传因素的影响,有可能与肿瘤细胞的恶性表型有关。
Objective To investigate the variations of mtDNA from high and low metastatic mouse hepatocarcinoma cell sublines Hca-F and Hca-P, and the relationship between mutations of mtDNA and carcinogenesis. Methods The variations of D-loop, ND3 and tRNA^Met＋Glu＋lle gene fragments of mtDNA from Hca-F and Hca-P cells were analyzed by PCR-RFLP and sequencing techniques. Results No amplification fragment length polymorphism and restriction fragment length polymorphism were observed in tRNA^Met＋Glu＋llt, ND3 and D-loop of mtDNA from the 2 cell sublines. Sequence difference between these 2 cell sublines were found in mtDNA D-loop region by sequencing. Conclusions Genetic alteration of mtDNA non-coding region in tumors, which may reflect the environmental and genetic influences operative during tumor progression, can be linked to their tumorigenic ohenotvoe.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第20期2030-2032,共3页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目(39900173)
重庆市自然科学基金资助项目(2005)~~
关键词
线粒体DNA
肝癌
肿瘤
变异
mitochondrial DNA
hepatocarcinoma
tumor
variation
