摘要
利用芽孢杆菌多效调控基因ｈｐｒ、ｄｅｑＱａ、ｄｅｇＲ、ｄｅｇＵ／５３２（Ｈｙ）等和枯草杆菌Ｋｉ－２－１３２自身的积生孢、感受态相关基因的协调作用，构建了系列的高表达遗传工程宿主。这些宿主对于ＡＰｒＥ都实现了较高水平的表达。其中，由ｈｐｒ和ｃｏｍ、ｓｐｏ等配合的宿主表达ＡｐｒＥ的能力比野生型宿主提高了１００—２００倍。
Using the cooperations between sporulation and competence-forming related genes both from other pleitropic regulatory genes of Bacillus, hpr, degQa, degU/S 32(Hy)and degR and from the Bacillus subtilis Ki-2-132. The series high-expressive genetical engineering host strains were constructed. Those strains could express the AprE at a quit high level. Among them, the strain which constructed by the cooperation of hpr, corn and spo etc., had the capacity for AprE-producing by 100-200 fold higher than that in the wild type host strain.
出处
《高技术通讯》
CAS
CSCD
1996年第6期46-51,共6页
Chinese High Technology Letters
基金
863计划
国家自然科学基金
