摘要
目的:探讨P53,P63和P73蛋白表达与胃腺癌临床病理特征之间的关系．方法:用免疫组织化学技术,检测72例胃腺癌及其癌旁正常组织中P53,P63和P73蛋白表达情况．所有研究对象均为湖北地区汉族人．其中,癌肿位于胃远端(胃窦、胃角)51例,胃近端(胃底、胃体)21例;肠型GAC 44例、弥漫型28例;高分化腺癌20例、中分化腺癌29例、低分化和未分化腺癌23例;TNM分期:Ⅰ和Ⅱ期13例,Ⅲ和Ⅳ期59例．结果:胃腺癌组织中P53,P63和P73蛋白阳性表达率均明显高于正常组织(X2=4．72,P<0．05; X2=5．51,P<0．05;X2=9．75,P<0．01);胃窦／胃角腺癌与胃底／胃体腺癌组织P53蛋白表达率无显著差异(P>0．05);在弥漫型胃腺癌中的表达率明显高于肠型胃腺癌(X2=4．68,P<0．05);在低分化腺癌与高中分化腺癌之间以及Ⅲ,Ⅳ期腺癌与Ⅰ,Ⅱ期胃腺癌之间的表达率的差异均有显著性(X2=7．06,P<0．05;X2=3．95, P<0．05)．P63蛋白在低分化腺癌组织中表达率明显高于高中分化腺癌(X2=7．36,P<0．05);在胃窦／胃角腺癌与胃底／胃体腺癌之间、在弥漫型与肠型胃腺癌之间、在Ⅰ,Ⅱ期胃腺癌与Ⅲ,Ⅳ期腺癌之间均无显著差异．P73蛋白在Ⅰ,Ⅱ期胃腺癌组织中的阳性表达率明显低于Ⅲ,Ⅳ期腺癌(X2=4．14,P<0．05),在胃窦／胃角腺癌与胃底／胃体腺癌之间、在弥漫型与肠型胃腺癌之间、在高、中及低分化胃腺癌之间均无显著差异．在P53蛋白阳性与阴性表达的胃腺癌之间,P63和P73蛋白阳性表达率的差异无显著性．结论:P53,P63和P73过度表达与胃腺癌的发生相关联,但并无交互作用．
AIM： To explore the relationships between the expressions of P53, P63 and P73 protein and the carcinogenesis of gastric adenocarcinoma （GAC）. METHODS： Immunohistochemistry method was used to detect the expressions of P53, P63 and P73 proteins in tissues from 72 GACs and adjacent normal tissues. RESULTS： The positive rates of P53, P63 and P73 expression in GAC were significantly higher than those in the normal tissues （χ^2= 4.72, P 〈 0.05; χ^2= 5.51, P 〈 0.05; χ^2= 9.75, P 〈 0.01）. The expression of P53 protein was distinctly higher in GAC of diffuse type than that in GAC of intestinal type （χ^2= 4,68, P 〈 0,05）, and marked differences were observed between lowlydifferentiated GAC and highly-, moderatelydifferentiated GAC （χ^2= 7.06, P 〈 0.05） as well as between GAC of stageⅠ, Ⅱand stage Ⅲ, Ⅳ（χ^2= 3.95, P 〈 0.05）. There was significant difference for P63 expression between lowly-differentiated GAC and highly-, moderately-differentiated GAC （X^2=7.36, P 〈 0.05）. The expression of P73 was significantly lower in patients of stage I and II than that of stage Ⅲ and Ⅳ （χ^2= 4.14, P 〈 0.05）. P73 expression was not correlated with the differentiation, tumor location and Lauren types. P63 and P73 expression were not markedly different between P53-positive and negative patients. CONCLUSION： The expression of P53, P63 and P73 are associated with the carcinogenesis of GAC, but no correlations exist among them.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第24期2416-2420,共5页
World Chinese Journal of Digestology
