摘要
目的了解氟中毒大鼠雌激素水平与生精细胞端粒酶表达的关系。方法将30只雄性SD大鼠随机分为对照组、低剂量染毒组、高剂量染毒组,分别按0、10和20mg/kg体重的染毒剂量隔日腹腔注射氟化钠(NaF)溶液39天后,用放射免疫法测定大鼠血清中雌二醇含量,用原位杂交法检测端粒酶逆转录酶(TERT)表达情况。同时镜检成熟精子质量和睾丸组织病理学改变。结果染毒各组大鼠血清雌二醇含量低于对照组(P<0·05);且随着染毒剂量的增加,雌二醇含量逐渐降低。染毒各组大鼠生精细胞TERT阳性表达率分别低于对照组(P<0·05);且随着染毒剂量的增加,TERT阳性表达率逐渐降低。各染毒组大鼠精子数量和精子存活率低于对照组(P<0·05);精子畸形率高于对照组(P<0·05);随着染毒剂量的增加,精子数量、精子存活率降低(P<0·05),精子畸形率升高(P<0·05)。各染毒组大鼠血清雌二醇含量与生精细胞TERT表达率均呈正相关,低剂量染毒组r=0·941,P<0·01;高剂量染毒组r=0·929,P<0·01。结论NaF很可能是通过雌激素/雌激素受体-端粒酶逆转录酶-精子途径对生殖系统造成损害的,各染毒组大鼠血清雌激素含量与生精细胞TERT表达率呈显著正相关。
Objective To study the relation between telomerase reverse transcriptase（TERT） activity expression in spermatogenic cells and serum levels of estradiol of fluorotic rats. Methods We randomly divided thirty SD male rats into control group, low-dose group and high-dose group, then inject sodium fluoride（0,10,20mg/kg bw）into celiac of rats. We respectively observed changes of estrogen and TERT using methods of radioimmunoassay,in situ hybridization. In addition, we observed the quality of spermatozoa. Results The level of estrogen,the expression of telomerase and the number and the livability of the spermatozoon in low-dose and high-dose fluorotic rats were lower than those of control rats（ P 〈 0.05）. Therefore, the above indexes decreased with the increase of dosage. In addition, sperm aberration of each fluorotic group was higher than control group （ P 〈 0.05）. And it increased with the increase of dosage. The content of E2 in serum of different fluoride treatment groups was positively correlated with the expression of telomerase in seminiferous tubule significantly, respectively （low-dose fluoride treatment groups, r = 0.941 , P 〈 0.01, high-dose fluoride treatment groups, r = 0.929, P 〈 0.01）. Conelusion NaF possibly damaged the male reproductive system by the approach of E2/ER-TERT-spermotozoon, relation between TERT expression in spermatogenic cells and serun levels of estradiol is positive correlation.
出处
《卫生研究》
CAS
CSCD
北大核心
2006年第5期546-548,共3页
Journal of Hygiene Research
基金
河南省科技攻关资助项目(No.524410007)
