摘要
目的研究三七总皂甙对大鼠脑出血后核转录因子κB(NF-κB)表达的影响。方法将SD大鼠随机分为正常组、假手术组、脑出血模型组、三七总皂甙治疗组,采用VII型胶原酶注入大鼠右侧苍白球制备脑出血模型,每组分别在术后6、24、48、96h4个时点收取脑组织标本,免疫组化检测NF-κBp65蛋白表达,同时测量脑组织含水量。结果正常组和假手术组比较,大鼠脑出血后脑组织NF-κBp65表达以及脑组织含水量显著增高(P<0·01);与脑出血模型组比较,三七总皂甙治疗组NF-κBp65水平(P<0·01)及脑组织含水量(6hP<0·05,24、48、96hP<0·01)均显著降低。结论三七总皂甙可以抑制脑出血后NF-κB表达,这可能是三七总皂甙减轻脑出血后脑水肿的机制之一。
Objective To study the influence of sanqizongzaodai on the expression of NF-κB in the perihematoma regions of rats brain. Methods Sixty-four Sprague Dawley male rats were randomly divided into normal group （A）, pseudo-operation group （B）, model group （C） and sanqizongzaodai treatment group （D）. ICH models of rats were induced by injection of V Ⅱtype collagenase into the right globas pallidus. The brain tissue specimen in each group were gathered at 6,24,48and 96 h after operation, respectively. The expression of NF-κB P65 in rat brain after ICH was detected with immunohistochemistry method,and at the same time the moisure in brain tissue was measured. Results Compared with group A and group B , the expression of NF-κBP65 and moisture in brain tissue in group C were obviously increased （ P〈0. 01）. Compared with group C , the expression of NF-κB P65 and moisture in brain tissue in group D were obviously decreased. Conclusion Sanqizongzaodai can reduce the expression of NF -κB in rat after ICH, which may be related with its anti-edema effect following ICH.
出处
《江苏医药》
CAS
CSCD
北大核心
2006年第10期941-942,F0003,共3页
Jiangsu Medical Journal
基金
南京医科大学科技发展基金项目(NY02033)
关键词
脑出血
核转录因子-ΚB
脑水肿
三七总皂甙
Intracerebral hemorrhage
Nuclear factor-κB
Brain edema
Sanqizongzaodai
