摘要
目的观察骨保护素配体(osteoprotegerin ligand,OPGL)在燃煤型氟中毒大鼠骨组织中的表达,(?)讨燃煤型氟中毒对大鼠OPGL表达的影响。方法以SD大鼠为研究对象,按体质量均衡随机分为5组(组内雌雄各半)：高氟组、高氟偏食组、低氟组、低氟偏食组、对照组(实验中所设偏食组为相对于对照组的低钙偏食)。以氟中毒病区煤烘玉米为主要饲料,复制氟中毒动物模型。对其胫骨干骺端进行常规组织切片,HE染色观察组织学改变；用免疫组织化学方法检测OPGL在大鼠胫骨干骺端的表达。氟离子选择电极法测定大鼠牙氟、骨氟。结果①OPGL表达水平：高氟组(118．62±1．27)、高氟偏食组(97．62±1．22)、低氟组(156．25±1．02)、低氟偏食组(145．25±1．25)大鼠干骺端OPGL表达增高,与对照组(189．23±1．25)比较差异有统计学意义(P＜0．05)。高氟偏食组与高氟组、低氟偏食组与低氟组比较,OPGL表达升高且差异有统计学意义(P＜0．05)。②骨病理改变：高氟、低氟组大鼠骨皮质厚度增加、密度增高,骨小梁成骨细胞数轻度增加,湿示成骨活跃：高氟偏食组大鼠胫骨骨皮质密质骨出现松质化。③骨密度：高氟偏食组大鼠骨密度低于对照组(P＜0．05),其余各实验组高于对照组,高氟组、低氟组与对照组比较差异有统计学意义(P＜0．05)。④牙氟、骨氟：各染氟组牙氟、骨氟均高于对照组(P＜0．05)。结论①过量氟造成骨转换增高,氟可通过增强OPGL的表达来加强破骨细胞的活性；②低钙偏食可使氟骨症破骨性骨吸收增强。
Objective To observe the expression of osteoprotegerin ligand （OPGL） in the bones of fluorosis rats caused by coal burning. Methods SD rats taken as the experimental objects were randomly assorted into 5 groups （the number of female or male was equal in each group）： control group, high-dose fluoride group, high fluorine with biased dietary group, low-dose fluorine group, low fluorine with biased dietary group. All rats were fed with corn dried by burning coal coming from endemic fluorosis areas with high fluoride, to establish the animal model of fluorosis. The tibia slices of chronic fluorosis rats were stained with HE; OPGL expressed in tibia metaphysis was observed by immunohistochemistry. Results ①Compared with control group, the number of OPGL increased in every test group was statistically significant（P〈0.05）. At the same level of fluorine, the increase of OPGL expression in biased dietary group is higher than that in normal diet group （P 〈0.05）. ②Pathologically, the thickness and density of the rat bone cortex in high-dose fluorine group increased. The osteoblast of trabecular bone tissue slightly increased; In high-dose fluorine with biased dietary bias group, the cartilage cells slightly increased in number and disarranged in order. The metaphyseal trabecular bone was covered by similar bone; The compact bone of the cortex become porosity and intenerate. ③Except the bone mineral density（BMD） in the high-dose fluorine with biased dietary group was lower than that in control group （P 〈 0.05）, BMD in every other high-dose fluorine group and low-dose fluorine group were higher than that in control group with a significant difference（P〈0.05）. ④The content of fluorine in bone of each test group increased, which was significant compared with control group （P〈0.05）. Groups with biased dietary bias had higher fluoride than these with normal diet group when they were fed same level of fluorine （P〈0.05）. Conclusions ① Excessive fluoride can induce accelerative bone turnover and enhance the activity of osteoclastic absorption by increasing the OPGL. ②iow calcium diet can promote bone absorption of skeletal fluorosis.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2006年第5期503-506,共4页
Chinese Jouranl of Endemiology
基金
贵州省科学技术基金重点项目[黔基金(2002)3011]
关键词
氟化物
中毒
煤
骨保护素配体
骨和骨组织
Fluorides
Poisoning
Coal
Osteoprotegerin ligand
Bone and bones
