摘要
目的探讨胚胎脊髓神经干细胞移植对大鼠脊髓损伤后神经功能恢复的意义。方法160只SD大鼠随机分为空白组,假手术组,脊髓损伤组,细胞移植组,分别在细胞移植后1、2、4周应用斜板实验和Tarlov评分对脊髓损伤后功能恢复进行评价,应用nestin标记观察移植后干细胞的存活情况。结果移植后1周、2周、4周,移植组和对照组斜板试验结果分别为(38．30±0．84)°、(18．50±0．76)°；jm(39．40±0．78)°、(19．70±0．66)°；(45．00±0．81)°、(22．30±0．69)°；Tarlov评分分别为3．37±0．45、2．32±0．34；3．45±0．38、2．41±0．43；3．63±0．47、2．45±0．48；有统计学意义(P＜0．01),免疫组织化学观察可见在损伤的脊髓组织中有神经干细胞的存活。结论胚胎脊髓干细胞移植对脊髓损伤后神经功能恢复有促进作用。
Objective To study the significance of rat spinal cord derived neural stem cell transplantation for the repair of transectional spinal cord injury （SCI）. Methods One hundred and sixty S-D rats approximately weighing 200-250 g were used for this study. The rats were randomized into cell transplantation group, sham operation group, simple injury group and vacant group. After the cells of spinal cord derived neural stem cell were separated and cultured in vitro to the fifth generation, the cells were transplanted onto the transectionally injured spinal cord. The rats in the simple injury group and sham operation group were injected with the same dose of saline as in the transplantation group. One,2,4 weeks after cell transplantation,the function was evaluated. Results At lst,2nd and 4th week after injury,the angles of tilting board in the transplantation and simple injury group were （38.30±0.84）°, （18.50±0.76）°; （39.40 ± 0.78）°, （19.70 ± 0.66）°; （45.00± 0.81）°, （22.30± 0.69）°. The Tarlov scores of hindlimb function in the transplantation and simple injury group were 3.37 ± 0.45,2.32 ± 0.34; 3.45 ± 0.38, 2.41 ± 0.43; 3.63 ± 0.47,2.45 ± 0.48 （P〈 0.01）. The functional recovery of spinal cord was better in the transplantation group than in the simple injury group. Conclusion The cultured spinal cord derived cells repaired the injured spinal cord to some extent.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2006年第10期1257-1258,共2页
Chinese Journal of Experimental Surgery
关键词
脊髓损伤
神经干细胞
细胞移植
Spinal cord injury
Neural stem cells
Cell transplantation
