摘要
目的:观察中等强度游泳运动后非酒精性脂肪性肝病大鼠模型肝脏组织的病理变化。方法:实验于2005-03/07在河北省医学科学院实验室完成,取雄性清洁级SD大鼠22只,随机分为3组:①对照组(n=6):给予普通饮食8周。②非酒精性脂肪性肝病模型组(n=8):喂高脂饲料,即在普通饲料的基础上加100g/L猪油和20g/L的胆固醇,共8周。③高脂运动组(n=8):喂高脂饲料,并进行中等强度的游泳运动,游泳5d/周,1次/d,90min/次,共8周。实验8周后测定各组大鼠血清谷草转氨酶和谷丙转氨酶活性和肿瘤坏死因子α水平,并比较各组大鼠肝脏组织切片的病理变化,光镜下评估肝细胞脂肪变性程度,按照肝小叶内含脂滴细胞数/总细胞数比值:0(-);<1/3(+);1/3～2/3(!);>2/3(");≈1($)。结果:实验过程中运动组1只大鼠溺水死亡。最后进入结果分析对照组、非酒精性脂肪性肝病模型组和运动组分别为6,8和7只大鼠。①非酒精性脂肪性肝病模型组和运动组大鼠肝细胞脂肪变性程度明显较对照组大鼠严重[非酒精性脂肪性肝病模型组、运动组和对照组脂肪变性程度-,+,!,"分别为0,0,3,5只;2,3,2,0只;6,0,0,0只(P<0.05)],与非酒精性脂肪性肝病模型组相比,运动组大鼠肝细胞脂肪变性程度明显减轻(P<0.05)。②非酒精性脂肪性肝病模型组大鼠血清谷丙转氨酶、谷草转氨酶都显著高于对照组([912.18±252.71,419.25±74.34;3281.82±776.32,2186.43±334.73)nkat/L(P<0.05)],运动组谷丙转氨酶显著低于非酒精性脂肪性肝病模型组([536.94±186.7)nkat/L(P<0.05)],但运动组谷草转氨酶与非酒精性脂肪性肝病模型组相比差异无显著性意义(P>0.05)。③3组大鼠血清肿瘤坏死因子α水平相比差异无显著性意义(P>0.05)。结论:中等强度的游泳运动能够改善高脂饮食造成的非酒精性脂肪性肝病的肝脏脂肪变性。
AIM： To observe pathological change of liver tissue in rat models with nonalcoholic fatty liver disease after moderate intensity swimming training.
METHODS： The experiment was performed at the laboratory of Acedemy of Medicine Science of Hebei Province from March to July 2005. Totally 22 clean-grade male SD rats were selected and assigned randomly into 3 groups： （1）Control group （n=6）. The rats received common diet for 8 weeks. （2）Nonalcoholic fatty liver disease group （n=8）： The rats were treated with high-fat feed, that was, adding 100 g/L pork fat and 20 g/L cholesterol for 8 weeks based on common feed. （3）High-fat-diet training group （n=8）： The rats were given high-fat feed and did moderate intensity swimming training, swimming for 5 days per week, once a day, 90 minutes every time for 8 weeks, and then the activities of serum glutamic-oxaloacetic tansaminase （SGOT） and glutamic pyruvic transamifiase （GPT） and level of tumor necrosis factor （TNF） α were measured. Pathological change of liver tissue section was compared in each group. Degree of hepatic steatosis was evaluated under light microscope, according to the ratio of number of lipid droplet of hepatic lobules and total number of cells： 0（-）;〈 1/3（＋）;1/3- 2/3（＋＋）;〉 2/3（＋＋＋）; ≈ 1 （＋＋＋＋）.
RESULTS： One rat in the high-fat-diet training group died of drowning in the trial. A total of 6, 8 and 7 rats in the control group, nonalcoholic fatty liver disease group and high-fat-diet training group, respectively were involved in the result analysis. （1）The hepatic steatosis in the nonalcoholic fatty liver disease group and high-fat-diet training group was more severe than that of the control group [hepatic steatosis -, ＋, ＋＋ , ＋＋＋ in the nonalcoholic fatty liver disease group, high-fat-diet training group and control group was 0,0,3,5 rats;2,3,2,0 rats;6,0,0,0 rats, respectively （P 〈 0.05 ）]. Compared with the nonalcoholic fatty liver disease group, the hepatic steatosis was significantly lighten in the high-fat-diet training group （P 〈 0.05）. （2）GPT and SGOT of rats in the nonalcoholic fatty liver disease group was obviously higher than those in the control group [（912.18±252.71,419.25±74.34;3 281.82±776.32,2 186.43±334.73） nkat/L （P 〈 0.05）]. The level of GPT of the high-fat-diet training group was markedly lower than that of the nonalcoholic fatty liver disease group [（536.94±186.7）nkat/L （P 〈 0.05）], but there was not remarkable difference in SGOT between the high-fat-diet training group and the nonalcoholic fatty liver disease group （P 〉 0.05）. （3）There was not remarkable difference in TNF α level of rats in the 3 groups （P 〉 0.05）.
CONCLUSION： The moderate swimming training can improve the nonalcoholic hepatic steatosis induced by high-fat diet.
出处
《中国临床康复》
CSCD
北大核心
2006年第40期103-105,F0003,共4页
Chinese Journal of Clinical Rehabilitation
