摘要
以亲水性两相分配法从发育菜豆子叶制备的质膜制剂经冻融循环操作，部分膜微囊可转变成密闭的翻转型。取冻融４次的质膜微囊用于Ｈ＋－ＡＴＰａｓｅ试验表明，ＡＴＰａｓｅ活力为ＡＢＡ和ＣａＭ显著地激活，但受ＩＡＡ显著抑制；质子泵活力被ＡＢＡ显著促进，但为ＣａＭ显著抑制，ＩＡＡ对质子泵活力无显著效应。可以认为：ＡＢＡ促进发育菜豆子叶吸收光合同化物可能是通过促进质膜Ｈ＋－ＡＴＰａｓｅ活力，从而促进质子／蔗糖同向运输而获得；ＩＡＡ则可能对菜豆子叶的质膜Ｈ＋－ＡＴＰａｓｅ无显著效应。在激素信号传导途径中，ＣａＭ对质膜Ｈ＋－ＡＴＰａｓｅ活力可能无直接影响。
Plasma membrane vesicles were purified from developing cotyledons of common beau by aqueous two-phase partitioning. Parts of these vesicles were turned into sealed, inside-out form by freeze/thaw cycle, and membrane vesicles frozen and thawed 4 times were routinely used in the H+-ATPase assay.It was observed that the ATPase activity was stimulated significantly by ABA and CaM but inhibited significantly by IAA; Proton pumping activity was Stimulated significantly by ABA but inhibited significantly by CaM, while IAA had only negligible effect on proton pumping. These findings imply that the enhancing effect of ABA on sugar uptake by developing beau cotyledons may be mediated through the stimulation of plasma membrane H+-ATPase; whereas LAA probably exerts no significant influence on plasma membrane H+ATPase in these tissues. CaM, a key link in the signal transduction pathway,might have no direct effect on plasma membrane H+-ATPase activity.
