摘要
用SDS-PAGE及免疫印渍法分析了三种镰孢的体外抗原(exoantigen)和菌丝体可溶性蛋白质的部分特性,并研究了培养基对体外蛋白质含量的影响。结果表明,在电泳分析中,三种镰孢体外抗原及菌丝体可溶性蛋白质均具有各自菌种的特征,可作为菌种分类鉴定的重要指标。免疫印渍分析显示,体外抗原更适于用作免疫分类鉴定的指标,因为用体外抗原免疫动物所产生的抗体的特异性比菌丝体可溶性蛋白质要好。三种镰孢的体外抗原的抗体与种间菌株均有程度不等的交叉反应,但却不与谷物发生任何交叉反应,可用于谷物中镰孢的快速检测。在镰孢体外抗原中,能刺激机体产生抗体的抗原分子量在28000以上。葡萄糖酵母膏培养基比蔗糖硫酸铵培养基更适于体外抗原的产生。
The partial characterization of exoantigens and mycelia soluble proteins from Fusarium were analyzed by SDS-PAGE and immunoblotting. The results showed that all three exoantigens and three mycelia soluble proteins from F. sporotrichioides, F. poae and F. graminearum have their own patterns differed from each other in gel. Immunoblotting analy- sis demonstrated that the exoantigens were more suitable for being used as an indicator for taxonomy because the specificity of antisera to exoantigens was better than that of antisera to mycelia soluble proteins. The antisera to exoantigens did not cross-react with extracts of grains but tended to have variable degree of cross-reactivities with exoantigens from different Fusarium species. The molecular weight of immunodominant antigens from Fusaria is above 28000.
出处
《微生物学报》
CAS
CSCD
北大核心
1996年第3期208-212,共5页
Acta Microbiologica Sinica
