摘要
已分离到一中等重复序列以及具有逆转座子样结构的元件Tcal(Transposon Candida albicans)。Tcal两端存在两个完全相同同向排列的序列LTR(Long Terminal Repeat)388bp,中间被一5.5kbDNA片段隔开。以alpha及Tcal为探针对40多种来自美国和中国的临床分离到的致病菌株进行杂交分析,根据杂交图谱,这些白色念株菌可被分成若干组,令人感兴趣的是来自同一地区菌种的遗传相关性比来自不同地区的大。比较URA3等基因的杂交结果,支持这种分析。尚未观察到alpha重复序列与其他酵母菌染色体DNA杂交的杂交条带,认为Tcal元件也许与C. albicans的遗传进化及其致病性有某种内在联系。
We had isolated from Candida albicans a moderately repetitive sequence designated alpha and a retrovirus-like transposable element Tcal( Transposon Candida albicans). The Tcal consisted of two 388bp direct repeats of the alpha element, called LTR(Long Ter- mination Repeat), which was separated by approximately 5.5kb of DNA. A large number of strains from America and China have been grouped based on patterns of hybridization bands visualized on Southern blots of EcoR I digested genomic DNA probed with alpha and Tcal element internal sequence. Strains from same area have higher relatedness than those from different area. The hybridization patterns with URA3 and other DNA probes were also conserved within the groups, alpha element are species specific, no hybridization was observed with genomic DNA of other yeast species. The data presented here indicate that the alpha element can be employed to distinguish between species and to assess strain relatedness within C.albicans, we suggest that Tcal may be relevant to the genomic evolutions of C.albicans and the pathogenic potential of the organism.
出处
《微生物学报》
CAS
CSCD
北大核心
1996年第3期161-167,共7页
Acta Microbiologica Sinica
基金
国家自然科学基金
上海市青年科技启明星计划资助
关键词
白色念珠菌
逆转座子样元件
分类鉴定
Candida albicans, alpha Repeat sequence, Tcal element, Taxonomic determina- tion
