摘要
目的探讨缺氧诱导因子在妊娠期肝内胆汁淤积症(intrahepaticcholestasisofpregnancy,ICP)患者胎盘上的表达及意义。方法通过体外缺氧/复氧培养胎盘绒毛组织,采用免疫组织化学染色技术,研究ICP患者和正常晚孕妇女胎盘上缺氧诱导因子(hypoxia-inducibletranscriptionfactor,HIF)HIF-1α,HIF-2α表达的变化。结果1ICP组缺氧(2%O2)4h与常氧(21%O2)下比较,HIF-1α表达明显增加(P<0.05)。复氧15min,两组HIF-1α均较缺氧4h的表达明显降低(P<0.05)。2正常组缺氧4h,HIF-2α的表达较常氧下明显升高(P<0.01);复氧15min,HIF-2α的表达较缺氧4h明显降低(P<0.01)。而缺氧/复氧过程中,ICP组HIF-2α的变化差异无显著意义(P>0.05)。结论ICP胎儿缺氧时,HIF-1α的应激性升高,对其适应缺氧有重要意义,可能是分子水平上ICP急性缺氧的敏感指标。急性缺氧时,ICP胎盘上HIF-2α的功能障碍可能导致不良妊娠结局。
Objectives We investigated the expression of the hypoxia-inducible transcription factor （HIF） in placenta of pregnant women with intrahepatic cholestasis of pregnancy（ICP）. Methods HIF-lα and HIF-2α Proteins from villous explant culture under hypoxia condition were probed by immuno- histochemistry methods. Results Q） HIF-1α protein in the placental villous tissues cultured for 4 h under hypoxic condition （2% O2） were detected in two groups. The expressions of HIF-1α increased in the placentae of pregnant women with ICP （P〈0. 05）. With the O2 concentration recovered, the high O2 concentration （21% O2） decreased the expression HIF-1α in 15 min （P〈0. 05） in two groups. ②The expression of HIF-2α was highly increased under hypoxia condition than that under normal concentration O2（P〈0.01）; 21% O2 decreased the expression HIF-2α in 15 rain （P〈0. 01） in normal group. But no significant difference was revealed in the change of the HIF-2α in ICP （P〈0. 05）. Conclusions Our research suggested that as a sensitive index, high increase of HIF-1α may play an important pole in adapting hypoxia and the abnormal change of HIF-2α may also bring bad pregnancy outcome in ICP under hypoxic conditions.
出处
《中华妇幼临床医学杂志（电子版）》
CAS
2006年第5期254-256,共3页
Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)
基金
国家教委博士点基金资助(编号:20020610030)
关键词
妊娠期肝内胆汁淤积症
缺氧诱导因子
intrahepatic cholestasis of pregnancy （ICP）
hypoxia-inducible transcription factor
