摘要
目的观察脑心通胶囊对血管性痴呆模型大鼠行为学及海马组织形态学的作用。方法采用大脑中动脉闭塞法(MCAO)制作血管性痴呆动物模型;跳台试验测定大鼠学习记忆成绩;取脑组织作冰冻切片,HE染色,观察大鼠海马形态学改变。结果跳台实验中,与假手术组比较,模型组大鼠存在着明显的学习记忆障碍;与模型组比较,脑心通胶囊、喜得镇两治疗组大鼠学习记忆能力得到改善,且两组相比无显著性差异。光镜观察显示,假手术组大鼠海马CA1区锥体细胞排列紧密整齐,无明显神经元脱失;模型组大鼠海马CA1区锥体细胞排列稀疏、紊乱,神经元脱失明显,可见胶质细胞增生,中、西药治疗组可明显减轻大鼠CA1区海马神经元脱失现象,使锥体细胞形态较正常,排列较整齐,接近假手术组。结论脑心通胶囊对血管性痴呆模型大鼠有治疗作用。
Objective To study the effects of Naoxintong capsule on behavior and hippocampal histomorphology in vascular dementia rats. Methods The vascular dementia model was established by occlusion of the middle cerebral artery in rats. The faculty of learning and memory were evaluated by step-down avoidance test. The brain tissues were removed to be made frozen sections and stained with HE,and then the hippocampal histomorphology was observed and analyzed by light microcope. Results The results of step - down avoidance test showed that compared with sham - operated group, model group presented significant learning and memory disorder; compared with model group, Naoxintong capsule group and Hydergine group exhibited improvement of learning and memory disability. Meanwhile, there existed no obvious difference between these two therapy groups. Under the light microscope, hippocampal CAI field of rats in the sham -operated group exhibited closely and neatly spaced pyramidal cells, and insignificant neuron loss;in the hippocampal CAI field of rats in the model group sparse and disturbed pyramidal cells ,noticeable neuron loss and neuroglial cell proliferation could be seen. Neuron loss reduced significantly in the hippocampal CAI pyramidal cell field of the two therapy group and cell morphology presented relative normal. Conclusion Naoxintong capsule has therapeutic effect on rat model of vascular dementia.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2006年第9期1674-1676,共3页
Lishizhen Medicine and Materia Medica Research
关键词
脑心通胶囊
大脑中动脉闭塞
血管性痴呆
学习记忆
海马
Naoxintong capsule
Middle cerebral artery occlusion
Vascular dementia
Learning and memory
Hippocampus
