摘要
组织化学染色是检测幽门螺杆菌较灵敏的方法，但迄今为止所有组织化学方法均不特异，特别是在细菌数量较少或经治疗后，更易造成假阴性结果。为此，我们建立了原位聚合酶链反应（ＰＣＲ）方法，使用它对胃组织内的幽门螺杆菌进行检测。结果发现，相对于传统检测方法，原位ＰＣＲ具有较高的灵敏度和特异性，特别适用于细菌数量较少或经治疗后细菌变形或崩解的病例的检测。
Although histochemical staining is a sensitive method for diagnosis of Helicobacter pylori (Hpy) infection, it is not specific. False positive diagnosis is possible due to contaminating flora, while treatment potentially causes false negative results by low number of organisms or altered morphology. We developed a non radioactive in situ PCR method for detection of Hpy and compared it with conventional methods for diagnosis of the infection. The conclusion is that in situ PCR provides a sensitive and specific method for detection of Hpy. It should be particularly useful for confirmation of infection in cases equivocal with other methods.
出处
《军事医学科学院院刊》
CSCD
北大核心
1996年第4期259-262,共4页
Bulletin of the Academy of Military Medical Sciences
关键词
幽门螺杆菌
胃炎
胃肿瘤
聚合酶链反应
Helicobacter pylori
gastritis
stomach neoplasm
polymerase chain reaction
