摘要
目的：采用体外培养法，观察神经生长因子（ＮＧＦ）在钙拮抗剂和细胞外不同Ｃａ＋＋浓度条件下对脊神经节突起生长的影响。方法：取８天胚龄的来亨鸡胚获取脊神经节，实验分为４组：ＮＧＦ组、ＮＧＦ＋ｖｅｒａｐａｍｉｌ组、ＮＧＦ＋Ｃａ＋＋组、单纯Ｃａ＋＋组，培养期间定时对脊神经节突起生长长度和密度进行观察。结果：ＮＧＦ＋４ｍｍｏｌ／ＬＣａ＋＋组突起生长明显优于其它组（Ｐ＜０．０１），ＮＧＦ组与ＮＧＦ＋ｖｅｒａｐａｍｉｌ组比较差异具有极显著性（Ｐ＜０．０１）。结论：ＮＧＦ促脊神经节突起生长效应具有显著的钙依赖性。
Objective:To observe the effects of nerve growth factor(NGF)on neurite outgrowth in dorsal ganglia root under conditions of calcium channel antagonist and varied extracellular calcium.Methods:8 days chicken embryos were used to obtain dorsal ganglia root.Four experimental groups were as follows:NGF group,NGF+verapamil group,NGF+Ca++group and Ca++group.The mean length and density of neurite outgrowth were measured.Results:The mean lergth and density of outgrowth in NGF+4mM Ca++group were significantly better than other groups,there was significant difference between NGF+ verapamil group and NGF group.Conclusion:Neurite outgrowth in dorsal ganglia root enhanced by NGF significantly depends on extracellular free calcium and calcium influx through plasma menbrane.
出处
《中华显微外科杂志》
CSCD
北大核心
1996年第4期283-285,共3页
Chinese Journal of Microsurgery
基金
国家自然科学基金
关键词
神经生长因子
神经突起生长
钙拮抗剂
Nerve growth factor,Neurite outgrowth,Calcium channel antagonist
