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应用聚合酶链反应检测支气管肺泡灌洗液中的乙肝病毒

APPLICATION OF POLYMERASE CHAIN REACTION IN DETECTING HEPATITIS B VIRUS IN BROUCHOAL-VEOLAR LAVAGE FLUID
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摘要 用聚合酶链反应(PCR)检测了45例肺科病人的支气管肺泡灌洗液(BALF)中乙肝病毒(HBV),同时对其中24例作血清HBV检测,发现BALF中HBV阳性率为26.67%(12/45)、血清HBV阳性率为41.67%(10/24);血清HBV阳性者其BALF方有可能检出HBV、血清HBV阴性者其BALF不能检出HBV,BALF中HBV脱氧核糖核酸(HBV-DNA)可以复制、有传染性。 Polymerare Chain Reaction(PCR)was used to detect hepatitis B virus(HBV)in thebrouchoalveolar lavage fluid(BALF)obtained from 45 patients admitted to the pulmonary de-partment.At the same time,serum HBV determination was performed in 24 of these pa-tients. It vvas found that HBV positive rate was 26.67%(12/45)in BALF,and 41.67%(10/24)in the serum. HBV positive BALF could only be seen in patients whose sera were HBV posi-tiveland HBV could not be found in BALF obtained from those patients whose sera were HBVnegative.HBV in BALF was infective.
出处 《广西医科大学学报》 CAS 1996年第2期45-46,共2页 Journal of Guangxi Medical University
关键词 聚合酶链反应 乙型肝炎病毒 肺泡灌洗液 polymerase chain reaction hepatitis B virus dcoxvribonucleic acid bronchoalveolarlavage fluid
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