摘要
作者获得１株特异的抗血吸虫成虫２０－２４ｋＤａ蛋白多肽表位的杂交瘤细胞株（ＳｊＡ１１１）。分泌ＭｃＡｂ为ＩｇＧ１，腹水效价１．２×１０６。将ＳｊＡ１１１ＭｃＡｂ－ＨＲＰ结合物用Ｄｏｔ－ＥＬＩＳＡ检测４９９例慢性血吸虫病患者的循环抗原（ＣＡｇ），阳性者３９０例（７８．１６％）；２５例肺吸虫病人，３６例华支睾吸虫病人及１３２例正常人血清中，仅１例肺吸虫病人出现交叉反应。ＣＡｇ的检出率和阳性血清滴度倒数的几何均值（ＧＭＲＴ）随着每ｇ粪便虫卵数（ＥＰＧ）的增加而递增。吡喹酮治疗后各期病人粪孵阴性者ＣＡｇ的转阴率：半年为７５．５０％（１１４／５１），１年为７３．６８％（８４／１１４），１年半为８８．０４％（１０３／１１７）。
A hybridoma cell line (Sj A111) secreting McAb of 20-24kDa polypiptide epitopes from adult worm of S. japonicum has been prepared. The immunoglobulin class of SjA111 McAb was IgG1. The titer of ascited containing McAb reached 1.2×106. 499 cases with chronic schistosomiasis were detected by Dot-ELISA using SjA111 McAb-HRP conjugate. Positive rate of CAg was 78.15% (390/499). Only 1 out 25 cases with paragonimiasis showed the cross reaction while other 36 cases with clonorchiasis and 132 normal individuals showed negative reaction. The detection rate and GRMT of CAg corresponded with the increase of EPG. Six months, 1 year and 1. 5 years after effective treatment. The negative conversion rate of CAg became 75.5% (114/151), 73.69% (84/114) and 88. 0% (103/117) respectively.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1996年第5期38-39,33,共3页
Chinese Journal of Zoonoses
基金
UNDP/WorldBack/WHO/TDK资助
