摘要
目的配制血糖即时检验室间质控品并做评价。方法准备肝素抗凝全血标本,取部分全血即刻分离血浆在日立7600-110全自动生化仪上测定作为对照组,剩余的全血联合加入多种抗糖酵解剂,并在室温(22OC)保存2、8、24、48小时后测定血中葡萄糖的浓度,在日立7600-110全自动生化仪、雅培MediSense○ROptiumTM快速血糖仪、强生SureStepTMPlus稳步型血糖仪、罗氏GLUCOTREND○R2乐康全血糖仪上测定,并将各仪器各时间段的结果作组间比较。结果含有复合抗酵解剂的全血标本,可使血中葡萄糖浓度在24小时内保持稳定,24小时内各时间段之间的结果差异无显著性意义(F=1.52,P>0.05),48小时的血糖结果有所下降,与对照组结果比较,有显著性差异(P<0.01)。结论自制的全血血糖室间质控品配制简单,血糖浓度24小时内保持稳定,且不会干扰快速血糖仪的测定,符合血糖即时检验室间质控要求。
Objective：To confect and evaluate the external quality control sample of Glucose concentration detection in point of care testing. Methods：Collected whole blood specimens with heparin as anticoagulant , took a part of whole blood to separate plasma which would be determined by HITACHI 7600-110 Automatic Analyzer for control group,added many kinds of antiglycolytic agents in different concentrations to the remanent blood, preserved at room temperature （22℃）,and determined blood glucose concentration at different time points （2,8,24,48h） by HITACHI 7600-110 Automatic Analyzer , MediSenseOptium^TM Blood Glucose Electrodes , LifeScan SureStep^TM Plus glucometer,Roche GLUCOTREND2 glucometer ,then compared with the results of glucose concentration detection at different time points. Results： the blood glucose concentration of whole blood specimens which contained mixture antiglycolytic agents kept stable ,the results at different points in 24h didn＇t have prominent significances（F= 1.52,P〉0.05） . The blood glucose concentrations declined after 48h , comparing with the results of control group, had prominent significances （P〈0. 01）. Conclusion： confecting the self-regulating whole blood external quality control sample is simple , glucose concentration of the whole blood external quality control sample can keep stable in 24h , furthermore the sample don＇t interfere the determining of glucose concentration by some glucometers that use extensively in the hospital POCT , the self-regulating whole blood external quality control sample accord with the request of the external quality control sample.
关键词
即时检验
快速血糖仪
室间质控品
制备
评价
Point-of Care Testing
Glucometer
the external quality control sample
preparation
evaluation
