摘要
目的:为探讨肝组织中糜酶(Chymase)浓度在慢性病毒性肝炎肝纤维化中的意义。方法:采用酶联免疫吸附实验(ELISA)检测45例慢性肝炎肝组织中Chymase浓度,观察不同的纤维化分期(S1-S4),其肝组织中Chymase浓度;用免疫组织化学染色法观察Chymase单克隆抗体标记的肥大细胞分布情况。结果:慢性肝炎纤维化分期重的S3和S4患者,其肝组织中Chymase浓度(S3+S4=31.3±24.6ng/mg)明显高于纤维化轻的S1和S2患者(S1+S2=5.7±4.8ng/mg),P<0.01;免疫组化染色结果,Chymase标记的肥大细胞主要分布于纤维化旺盛的汇管区与类洞壁,其分布与纤维化部位相一致,且肝组织中Chymase浓度高的患者,其肝组织内Chymase标记的肥大细胞分布增多。结论:肝组织中Chymase浓度可能与慢性肝炎肝纤维化关系密切。
Objective：To investigate spatial relationship between human chymase and Fibrosis in liver specimens affected by chronic hepatitis. Methods ：The chymase levels were determined by enzyme-linked immunosorbant assay（ELISA） in 45 cases of chronic hepatitis. Mast cell distribution in the specimens was determined immunohistochemically using anti-chymase antibudy. Results：Hepatic chymase concentrations in S3 and S4 cases were greater than in S1 and S2 （ S3 ＋ S4 = 31.3 ＋ 24. 6 ng/mg vs S1 ＋ S2 = 5.7 ＋ 4. 8 ng/mg; P 〈 0.01 ）. Cells immunoreacfive for chymase were seen throughout portal areas and intralobular sinusoidal walls, largely colocalizing with fibrosis. Conclusion：Chymase appears to be involved in hepatic fibrosis in chronic hepatitis.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2006年第9期876-877,共2页
Chinese Journal of Immunology
