摘要
目的Z-HL16C建株后对其性质和应用的研究。方法将该细胞传代冻存再复苏、扩大应用，进而对其性质进行鉴定。结果类上皮细胞，形态均一、界限清晰、有立体感。经传代、培养、冻存至今已10年，复苏率90％以上。该细胞株对脊髓灰质炎，柯萨奇A-B，艾柯病毒，甲、乙型流感病毒，1～3型副流感病毒，合胞病毒，麻疹病毒，风疹病毒，腺病毒（2，3，7，11型），1—2型单纯疱疹病毒，水痘带状疱疹病毒及巨细胞病毒均敏感，经鉴定为癌变细胞系。结论Z-HL16C为稳定的、对病毒广谱敏感的癌变细胞系。
Objective To establish and study the nature and the application of Z-HL16C cell line. Methods The cell line was continuously passed, frozen stored and recovered. Its application was expanded and the cell type was identified. Result The cell line had an epithelial-cell-like shape,the size appeared uniform,the cell boundary was distinct. It has been continuously passed, frozen stored and recovered for ten years. Its recovery rate was about 90% . It has been proved to be sensitive to the tested viruses which were enteroviruses （Polio,Cox, Echo）, influenza viruses, parainfluenzaviruses, adenoviruses, measles virus. This cell line has been identified as a cancerization cell. Conclusion The cell line Z-HL16C has been stably established, it has a broad spectrum in sensitivity for culturing viruses.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2006年第3期232-234,共3页
Chinese Journal of Experimental and Clinical Virology
